Hsp70 expression in monocytes from patients with peripheral arterial disease and healthy controls: monocyte Hsp70 in PAD
Hsp70 expression in monocytes from patients with peripheral arterial disease and healthy controls: monocyte Hsp70 in PAD
Background: heat shock proteins (HSP) are induced during cellular stress. Their role is to chaperone cellular proteins giving protection from denaturation and ultimately preventing cell death. Monocytes are key cells involved in atherosclerosis and are highly responsive to HSP induction. Therefore, we wished to examine monocyte Hsp70 expression and induction in patients with peripheral arterial disease (PAD) and in healthy controls.
Methods: we measured cellular Hsp70 levels in freshly isolated monocytes and released Hsp70 levels in plasma and monocyte culture supernatants, obtained from patients with PAD and from healthy controls. We assessed the effect of statin therapy on Hsp70 levels and examined monocyte cell survival in culture with and without immunological stress.
Results Monocyte cellular Hsp70 was lower in patients with PAD compared to healthy controls (11.3?±?7.4 ng/106 cells vs 20.7?±?16.0 ng/106 cells; p? <?0.001). Individuals on statin therapy from both PAD and control groups had lower monocyte Hsp70 compared to those not treated with statins. Concentrations of Hsp70 released into culture supernatants were not dependent on PAD or statin therapy. Cell survival was inversely associated with Hsp70 concentrations in culture supernatants but had no association with cellular concentrations of Hsp70.
Conclusions: cellular Hsp70 and released Hsp70 may play different roles in monocyte health. Whilst induced Hsp70 destined for release appears to be unaffected in PAD, mechanisms responsible for cellular retention of Hsp70 may provide an area for future therapeutic targets in vascular disease.
cell survival, Hsp70, monocyte, PAD, statin, inflammation
215-223
Madden, Jacqueline
0771e352-d432-41ea-8a7e-4704c1efca46
Coward, Jenifer C.
952245fa-1b59-4997-98ec-8529bd4aed4a
Shearman, Cliff P.
cf4d6317-f54d-4ab3-ba49-c6797897bbcf
Grimble, Robert F.
3100e4d2-8f29-4ca6-a95d-38a6a764865f
Calder, Philip C.
1797e54f-378e-4dcb-80a4-3e30018f07a6
2010
Madden, Jacqueline
0771e352-d432-41ea-8a7e-4704c1efca46
Coward, Jenifer C.
952245fa-1b59-4997-98ec-8529bd4aed4a
Shearman, Cliff P.
cf4d6317-f54d-4ab3-ba49-c6797897bbcf
Grimble, Robert F.
3100e4d2-8f29-4ca6-a95d-38a6a764865f
Calder, Philip C.
1797e54f-378e-4dcb-80a4-3e30018f07a6
Madden, Jacqueline, Coward, Jenifer C., Shearman, Cliff P., Grimble, Robert F. and Calder, Philip C.
(2010)
Hsp70 expression in monocytes from patients with peripheral arterial disease and healthy controls: monocyte Hsp70 in PAD.
Cell Biology and Toxicology, 26 (3), .
(doi:10.1007/s10565-009-9134-x).
(PMID:19672680)
Abstract
Background: heat shock proteins (HSP) are induced during cellular stress. Their role is to chaperone cellular proteins giving protection from denaturation and ultimately preventing cell death. Monocytes are key cells involved in atherosclerosis and are highly responsive to HSP induction. Therefore, we wished to examine monocyte Hsp70 expression and induction in patients with peripheral arterial disease (PAD) and in healthy controls.
Methods: we measured cellular Hsp70 levels in freshly isolated monocytes and released Hsp70 levels in plasma and monocyte culture supernatants, obtained from patients with PAD and from healthy controls. We assessed the effect of statin therapy on Hsp70 levels and examined monocyte cell survival in culture with and without immunological stress.
Results Monocyte cellular Hsp70 was lower in patients with PAD compared to healthy controls (11.3?±?7.4 ng/106 cells vs 20.7?±?16.0 ng/106 cells; p? <?0.001). Individuals on statin therapy from both PAD and control groups had lower monocyte Hsp70 compared to those not treated with statins. Concentrations of Hsp70 released into culture supernatants were not dependent on PAD or statin therapy. Cell survival was inversely associated with Hsp70 concentrations in culture supernatants but had no association with cellular concentrations of Hsp70.
Conclusions: cellular Hsp70 and released Hsp70 may play different roles in monocyte health. Whilst induced Hsp70 destined for release appears to be unaffected in PAD, mechanisms responsible for cellular retention of Hsp70 may provide an area for future therapeutic targets in vascular disease.
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Submitted date: 13 May 2009
Accepted/In Press date: 27 July 2009
Published date: 2010
Keywords:
cell survival, Hsp70, monocyte, PAD, statin, inflammation
Organisations:
Dev Origins of Health & Disease
Identifiers
Local EPrints ID: 141494
URI: http://eprints.soton.ac.uk/id/eprint/141494
ISSN: 0742-2091
PURE UUID: 3b5601d2-7df9-4e90-9788-1334841dbe01
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Date deposited: 30 Mar 2010 08:20
Last modified: 14 Mar 2024 02:39
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Author:
Jacqueline Madden
Author:
Jenifer C. Coward
Author:
Robert F. Grimble
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