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Micro fluidic cytometry for analysis of marine microorganisms

Micro fluidic cytometry for analysis of marine microorganisms
Micro fluidic cytometry for analysis of marine microorganisms
Knowledge of the distribution and concentration of phytoplankton (microscopic algae that live in the oceans) is important, as this reflects and may modify environmentally important events such as pollution, climate change and carbon dioxide sequestration. Optical based flow cytometry has proved to be strategically important for the study of marine environments in general and of phytoplankton in particular because it enables rapid, simultaneous quantitative analysis of multiple optical properties of particles. Unfortunately, cost, complexity and size limit the widespread use of this technique for the study and monitoring of phytoplankton.

The objective of this research was to solve some of the above mentioned problems by developing a small device for deployment on remote underwater vehicles (RUV) for in situ flow cytometric analysis of marine organisms. The core of the system was a lab-on-a-chip (LOC) device with a microfluidic channel with integrated optics and electrodes for the detection of the optical and electrical characteristics of phytoplankton.

The work describes the design, fabrication, characterisation and testing of various LOC devices, together with data and analysis of results. The devices were able to clearly distinguish different populations of phytoplankton and other micro-particles on the basis of their optical and electrical properties. The systems had performance comparable with commercial instruments.

Research also included bio-physical measurements (electro rotation and dielectrophoresis) of marine organisms to allow further modelling of the information obtained from the LOC cytometer.
Benazzi, Giuseppe
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Benazzi, Giuseppe
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Morgan, Hywel
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Mowlem, Matthew C.
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Benazzi, Giuseppe (2010) Micro fluidic cytometry for analysis of marine microorganisms. University of Southampton, School of Electronics and Computer Science, Doctoral Thesis, 210pp.

Record type: Thesis (Doctoral)

Abstract

Knowledge of the distribution and concentration of phytoplankton (microscopic algae that live in the oceans) is important, as this reflects and may modify environmentally important events such as pollution, climate change and carbon dioxide sequestration. Optical based flow cytometry has proved to be strategically important for the study of marine environments in general and of phytoplankton in particular because it enables rapid, simultaneous quantitative analysis of multiple optical properties of particles. Unfortunately, cost, complexity and size limit the widespread use of this technique for the study and monitoring of phytoplankton.

The objective of this research was to solve some of the above mentioned problems by developing a small device for deployment on remote underwater vehicles (RUV) for in situ flow cytometric analysis of marine organisms. The core of the system was a lab-on-a-chip (LOC) device with a microfluidic channel with integrated optics and electrodes for the detection of the optical and electrical characteristics of phytoplankton.

The work describes the design, fabrication, characterisation and testing of various LOC devices, together with data and analysis of results. The devices were able to clearly distinguish different populations of phytoplankton and other micro-particles on the basis of their optical and electrical properties. The systems had performance comparable with commercial instruments.

Research also included bio-physical measurements (electro rotation and dielectrophoresis) of marine organisms to allow further modelling of the information obtained from the LOC cytometer.

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Published date: January 2010
Organisations: University of Southampton

Identifiers

Local EPrints ID: 141969
URI: http://eprints.soton.ac.uk/id/eprint/141969
PURE UUID: f0cd55d3-c473-4611-824e-7d6ea0cf3a4c
ORCID for Hywel Morgan: ORCID iD orcid.org/0000-0003-4850-5676
ORCID for Matthew C. Mowlem: ORCID iD orcid.org/0000-0001-7613-6121

Catalogue record

Date deposited: 14 Jun 2010 15:32
Last modified: 14 Mar 2024 02:48

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Contributors

Author: Giuseppe Benazzi
Thesis advisor: Hywel Morgan ORCID iD
Thesis advisor: Matthew C. Mowlem ORCID iD

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