The use of an electroactive marker as a SERS label in anE-melting mutation discrimination assay
Mahajan, Sumeet, Richardson, James, Gaied, Nouha Ben, Zhao, Zhengyun, Brown, Tom and Bartlett, Philip N. (2009) The use of an electroactive marker as a SERS label in anE-melting mutation discrimination assay. Electroanalysis, 21, (20), 2190-2197. (doi:10.1002/elan.200904681).
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In this paper we describe the first example of the use of an electroactive label as a SERS marker for detecting and quantifying target oligonucleotides and distinguishing mutations using a combination of voltammetry and electrochemically induced melting (E-melting). The experiments were carried out on sphere segment void substrates selected to show strong surface enhancement for SERS at 633 nm. DNA analysis was carried out for CFTR sequences using synthetic 22-mer oligonucleotides labelled with a modified anthraquinone at the 3-end. Discrimination between the wild type, 1653C/T (point mutation) and F508 (triple deletion) is demonstrated.
|Subjects:||Q Science > QD Chemistry|
|Divisions:||University Structure - Pre August 2011 > School of Chemistry
|Date Deposited:||15 Apr 2010 15:21|
|Last Modified:||02 Mar 2012 11:34|
|Contributors:||Mahajan, Sumeet (Author)
Richardson, James (Author)
Gaied, Nouha Ben (Author)
Zhao, Zhengyun (Author)
Brown, Tom (Author)
Bartlett, Philip N. (Author)
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