Shotgun proteomic analysis of human-induced sputum
Shotgun proteomic analysis of human-induced sputum
Induced sputum is a readily accessible biological fluid whose composition may alter as a consequence of disease. To date, however, the proteins that routinely populate this biofluid are largely unknown, in part due to the technical difficulties in processing such mucin-rich samples. To provide a catalogue of sputum proteins, we have surveyed the proteome of human-induced sputum (sputome). A combination of 2-D gel analysis and GeLC-MS/MS allowed a total of 191 human proteins to be confidently assigned. In addition to the expected components, several hitherto unreported proteins were found to be present, including three members of the annexin family, kallikreins 1 and 11, and peroxiredoxins 1, 2 and 5. Other sets of proteins identified included four proteins previously annotated as hypothetical or conserved hypothetical. Taken together, these data represent the first extensive survey of the proteome of induced sputum and provide a platform for future identification of biomarkers of lung disease.
2-D PAGE, GeLC-MS/MS, human bronchoalveolar lavage fluid proteins, induced sputum
4390-4401
Nicholas, Ben
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Skipp, Paul
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Mould, Richard
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Rennard, S.
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Davies, Donna E.
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O'connor, D.
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Djukanovic, Ratko
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August 2006
Nicholas, Ben
785c44fb-6536-4189-803b-4545425e9385
Skipp, Paul
1ba7dcf6-9fe7-4b5c-a9d0-e32ed7f42aa5
Mould, Richard
4f405a64-202d-4e3f-830c-65128e7f3da3
Rennard, S.
5ed888ed-6c3d-47d8-8535-99089c150c0b
Davies, Donna E.
7de8fdc7-3640-4e3a-aa91-d0e03f990c38
O'connor, D.
c4910888-1df5-4be7-8142-af0e919f9100
Djukanovic, Ratko
d9a45ee7-6a80-4d84-a0ed-10962660a98d
Nicholas, Ben, Skipp, Paul, Mould, Richard, Rennard, S., Davies, Donna E., O'connor, D. and Djukanovic, Ratko
(2006)
Shotgun proteomic analysis of human-induced sputum.
Proteomics, 6 (15), .
(doi:10.1002/pmic.200600011).
Abstract
Induced sputum is a readily accessible biological fluid whose composition may alter as a consequence of disease. To date, however, the proteins that routinely populate this biofluid are largely unknown, in part due to the technical difficulties in processing such mucin-rich samples. To provide a catalogue of sputum proteins, we have surveyed the proteome of human-induced sputum (sputome). A combination of 2-D gel analysis and GeLC-MS/MS allowed a total of 191 human proteins to be confidently assigned. In addition to the expected components, several hitherto unreported proteins were found to be present, including three members of the annexin family, kallikreins 1 and 11, and peroxiredoxins 1, 2 and 5. Other sets of proteins identified included four proteins previously annotated as hypothetical or conserved hypothetical. Taken together, these data represent the first extensive survey of the proteome of induced sputum and provide a platform for future identification of biomarkers of lung disease.
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Published date: August 2006
Keywords:
2-D PAGE, GeLC-MS/MS, human bronchoalveolar lavage fluid proteins, induced sputum
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Local EPrints ID: 146555
URI: http://eprints.soton.ac.uk/id/eprint/146555
ISSN: 1615-9853
PURE UUID: 15e5a337-0b71-4ccb-936d-ad0f2e873e8b
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Date deposited: 11 Jun 2010 14:50
Last modified: 18 Mar 2024 02:58
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Author:
Ben Nicholas
Author:
Richard Mould
Author:
S. Rennard
Author:
D. O'connor
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