Direct demonstration of a Staphylococcus biofilm in an external ventricular drain in a patient with a history of recurrent ventriculoperitoneal shunt failure
Stoodley, P., Braxton, Jr. E.E., Nistico, L., Hall-Stoodley, L., Johnson, S., Quigley, M., Post, J.C., Ehrlich, G.D. and Kathju, S. (2010) Direct demonstration of a Staphylococcus biofilm in an external ventricular drain in a patient with a history of recurrent ventriculoperitoneal shunt failure. Pediatric Neurosurgery, 46, (2), 127-132. (doi:10.1159/000319396).
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External ventricular drains (EVD) are associated with a high infection rate. Early detection of infection is frequently problematic due to a lack of clinical signs and the time period required for culturing. Bacterial biofilms have been suggested to play an important role in the infection of EVD, but direct evidence is as yet lacking.
We report the case of a 17- year-old male with Dandy-Walker malformation who presented with headache, nausea and drowsiness; a CT scan revealed enlarged ventricles. The patient had a history of ventriculoperitoneal shunt revision 3 weeks prior to admission. The shunt was removed on suspicion of infection and an EVD placed. Daily surveillance cultures through the EVD were negative and the EVD was replaced on day 5. Examination of the initial EVD by confocal microscopy demonstrated clear intraluminal biofilm formation; molecular analysis by PCR identified Staphylococcus aureus resident on the catheter.
To our knowledge, this is the first direct demonstration of an intraluminal biofilm compromising an EVD. Despite the presence of biofilm on this catheter, the patient demonstrated no clinical signs of infection, and the routine surveillance culture was negative. Undetected biofilm may pose a latent risk on EVD and other neurosurgical catheters.
|Digital Object Identifier (DOI):||doi:10.1159/000319396|
|Additional Information:||Affiliation Paul Stoodley: The National Centre for Advanced Tribology at Southampton (nCATS)|
|Subjects:||R Medicine > RD Surgery
Q Science > QH Natural history > QH301 Biology
|Divisions :||University Structure - Pre August 2011 > School of Engineering Sciences > Engineering Materials & Surface Engineering
|Accepted Date and Publication Date:||
|Date Deposited:||30 Jul 2010 08:11|
|Last Modified:||31 Mar 2016 13:22|
|RDF:||RDF+N-Triples, RDF+N3, RDF+XML, Browse.|
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