Impact of ovarian stimulation on mid-luteal endometrial tissue and secretion markers for receptivity

van der Gaast, M.H., Classen-Linke, I., Krusche, C.A., Beier-Hellwig, K., Fauser, B.C., Beier, H.M. and Macklon, N.S. (2008) Impact of ovarian stimulation on mid-luteal endometrial tissue and secretion markers for receptivity. Reproductive BioMedicine Online, 17 (4), 553-563. (doi:10.1016/S1472-6483(10)60244-1).

Abstract

The objective of this study was to investigate the effect of ovarian stimulation for IVF on endometrial secretion and tissue markers of receptivity in the mid-luteal phase. In 10 oocyte donors, endometrial secretions and biopsies were sampled 5 days after spontaneous ovulation and oocyte retrieval in consecutive cycles. Four subjects received progesterone in the luteal phase of the stimulated cycles.

Mid-luteal endometrial maturation in the stimulated cycle was compared with the spontaneous cycle, by histological dating, Ki-67, oestrogen receptor (ER) and progesterone receptor (PR) expression, secretion levels of leukaemia inhibitory factor (LIF), glycodelin A (GdA) and progesterone, and protein profile. No significant differences in histological markers, expression of Ki-67, PR, ER, secretion protein profiles or concentrations of LIF, GdA, or progesterone were observed when comparing natural with stimulated cycles.

Progesterone supplementation of stimulated cycles was associated with significantly lower Ki-67 (P = 0.03) and ER (P = 0.04) expression compared with the non-supplemented stimulated cycle. In this pilot study, ovarian stimulation was not demonstrated to alter the studied markers of endometrial maturation in the mid-luteal phase.

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