Formation of a human-derived fat tissue layer in P(DL)LGA hollow fibre scaffolds for adipocyte tissue engineering
Formation of a human-derived fat tissue layer in P(DL)LGA hollow fibre scaffolds for adipocyte tissue engineering
Development of adipose tissue-engineering strategies, where human bone marrow stromal cells (HBMSC) are combined with three-dimensional scaffolds, is likely to prove valuable for soft tissue restoration. In this study, we assessed the function of poly(dl-lactide-co-glycolide) (PdlLGA) hollow fibres in facilitating the development of HBMSC-derived adipocytes for advancement of an associated adipocyte layer.
The large surface area of 75:25 PdlLGA fibres facilitated the rapid generation of extensive adipocyte aggregates from an undifferentiated HBMSC monolayer, where the fat-laden cells stained positive with Oil Red O and expressed the adipocyte marker, fatty acid binding protein 3 (FABP3). Following implantation subcutaneously in severely compromised immunodeficient mice, the adipogenic phenotype of the PLGA–adipocyte graft was maintained for up to 56 days.
Confocal microscopy showed associated LipidTOX™ Deep Red neutral lipid staining in an FLPdlLGA fibre–adipocyte graft after 56 days, critical evidence demonstrating maintenance of the adipocyte phenotype in the subcutaneous graft. To support adipose tissue advancement in a defined volume, the PdlLGA–adipocyte scaffold was encapsulated within alginate/chitosan hydrogel capsules (typical diameters, 4.0 mm). In a 28-day in vivo trial in immunodeficient mice, clusters of the capsules were maintained at the subcutaneous site. An adipocyte tissue layer advancing within the surrounding hydrogel was demonstrated.
human bone marrow stromal cells, adipocytes, soft tissue engineering, adipogenesis, poly(lactide-co-glycolide) hollow fibres, alginate/chitosan hydrogels
1910-1917
Morgan, Suzanne M.
e8af487a-f03a-43a5-a0f4-7093b508434b
Ainsworth, Ben J.
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Kanczler, Janos M.
eb8db9ff-a038-475f-9030-48eef2b0559c
Babister, Jodie C.
3b35e463-b623-403c-91c3-88a590cea674
Chaudhuri, Julian B.
40daebdf-c69f-486d-a4d8-a07f4789a40d
Oreffo, Richard O. C.
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April 2009
Morgan, Suzanne M.
e8af487a-f03a-43a5-a0f4-7093b508434b
Ainsworth, Ben J.
cc12c780-3c33-4a14-a5f2-32fe9cc2e937
Kanczler, Janos M.
eb8db9ff-a038-475f-9030-48eef2b0559c
Babister, Jodie C.
3b35e463-b623-403c-91c3-88a590cea674
Chaudhuri, Julian B.
40daebdf-c69f-486d-a4d8-a07f4789a40d
Oreffo, Richard O. C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Morgan, Suzanne M., Ainsworth, Ben J., Kanczler, Janos M., Babister, Jodie C., Chaudhuri, Julian B. and Oreffo, Richard O. C.
(2009)
Formation of a human-derived fat tissue layer in P(DL)LGA hollow fibre scaffolds for adipocyte tissue engineering.
Biomaterials, 30 (10), .
(doi:10.1016/j.biomaterials.2008.12.033).
(PMID:19135718)
Abstract
Development of adipose tissue-engineering strategies, where human bone marrow stromal cells (HBMSC) are combined with three-dimensional scaffolds, is likely to prove valuable for soft tissue restoration. In this study, we assessed the function of poly(dl-lactide-co-glycolide) (PdlLGA) hollow fibres in facilitating the development of HBMSC-derived adipocytes for advancement of an associated adipocyte layer.
The large surface area of 75:25 PdlLGA fibres facilitated the rapid generation of extensive adipocyte aggregates from an undifferentiated HBMSC monolayer, where the fat-laden cells stained positive with Oil Red O and expressed the adipocyte marker, fatty acid binding protein 3 (FABP3). Following implantation subcutaneously in severely compromised immunodeficient mice, the adipogenic phenotype of the PLGA–adipocyte graft was maintained for up to 56 days.
Confocal microscopy showed associated LipidTOX™ Deep Red neutral lipid staining in an FLPdlLGA fibre–adipocyte graft after 56 days, critical evidence demonstrating maintenance of the adipocyte phenotype in the subcutaneous graft. To support adipose tissue advancement in a defined volume, the PdlLGA–adipocyte scaffold was encapsulated within alginate/chitosan hydrogel capsules (typical diameters, 4.0 mm). In a 28-day in vivo trial in immunodeficient mice, clusters of the capsules were maintained at the subcutaneous site. An adipocyte tissue layer advancing within the surrounding hydrogel was demonstrated.
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Published date: April 2009
Keywords:
human bone marrow stromal cells, adipocytes, soft tissue engineering, adipogenesis, poly(lactide-co-glycolide) hollow fibres, alginate/chitosan hydrogels
Organisations:
Dev Origins of Health & Disease
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Local EPrints ID: 151741
URI: http://eprints.soton.ac.uk/id/eprint/151741
ISSN: 0142-9612
PURE UUID: bef0bd4f-ab4e-4fb1-8179-9625c94baee1
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Date deposited: 12 May 2010 10:44
Last modified: 14 Mar 2024 02:50
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Contributors
Author:
Suzanne M. Morgan
Author:
Ben J. Ainsworth
Author:
Janos M. Kanczler
Author:
Jodie C. Babister
Author:
Julian B. Chaudhuri
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