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Augmentation of skeletal tissue formation in impaction bone grafting using vaterite microsphere biocomposites

Augmentation of skeletal tissue formation in impaction bone grafting using vaterite microsphere biocomposites
Augmentation of skeletal tissue formation in impaction bone grafting using vaterite microsphere biocomposites
The development of particulate bone void fillers with added biological function to augment skeletal tissue formation will lead to improved efficacy in bone replacement surgery. We demonstrate the potential for vaterite microsphere biocomposites to augment bone matrix formation within an in vivo model for impaction bone grafting seeded with human bone marrow stromal cells. In vitro tests demonstrate the significance of vaterite microspheres in the activation and promotion of 3D skeletal tissue formation. Further in vitro experiments using functionalized microspheres with surface integrated RGD peptide activate co-cultured skeletal populations in pellets and promote secretion of extracellular matrix collagens and human osteocalcin. Specific temporal release of entrapped RNase A was successfully demonstrated using these specialized microspheres with integrated magnetic beads, which physically disrupted the inorganic macrostructure. These studies demonstrate that bio-inspired calcium carbonate microspheres augment in vivo bone formation in impaction bone grafting. Such microspheres with added biological functionality offer innovative therapeutic approaches to activate skeletal populations and enhance bone formation with reparative implications for hard tissues.

human osteoprogenitor, calcium carbonate, osteogenesis, biomimetic material, mineralization, bone tissue engineering
0142-9612
1918-1927
Green, David W.
9591e846-aeac-4549-b389-1744c5a43cff
Bolland, Benjamin J.R.
1290b975-6eaa-46b8-8dd0-0fb566ad2019
Kanczler, Janos M.
eb8db9ff-a038-475f-9030-48eef2b0559c
Lanham, Stuart A.
28fdbbef-e3b6-4fdf-bd0f-4968eeb614d6
Walsh, Dominic
f5bd569c-0316-4af1-af81-6cc606f10724
Mann, Stephen
1cbb2d4f-aee2-403c-9950-18bd388f02c0
Oreffo, Richard O.C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Green, David W.
9591e846-aeac-4549-b389-1744c5a43cff
Bolland, Benjamin J.R.
1290b975-6eaa-46b8-8dd0-0fb566ad2019
Kanczler, Janos M.
eb8db9ff-a038-475f-9030-48eef2b0559c
Lanham, Stuart A.
28fdbbef-e3b6-4fdf-bd0f-4968eeb614d6
Walsh, Dominic
f5bd569c-0316-4af1-af81-6cc606f10724
Mann, Stephen
1cbb2d4f-aee2-403c-9950-18bd388f02c0
Oreffo, Richard O.C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778

Green, David W., Bolland, Benjamin J.R., Kanczler, Janos M., Lanham, Stuart A., Walsh, Dominic, Mann, Stephen and Oreffo, Richard O.C. (2009) Augmentation of skeletal tissue formation in impaction bone grafting using vaterite microsphere biocomposites. Biomaterials, 30 (10), 1918-1927. (doi:10.1016/j.biomaterials.2008.12.052).

Record type: Article

Abstract

The development of particulate bone void fillers with added biological function to augment skeletal tissue formation will lead to improved efficacy in bone replacement surgery. We demonstrate the potential for vaterite microsphere biocomposites to augment bone matrix formation within an in vivo model for impaction bone grafting seeded with human bone marrow stromal cells. In vitro tests demonstrate the significance of vaterite microspheres in the activation and promotion of 3D skeletal tissue formation. Further in vitro experiments using functionalized microspheres with surface integrated RGD peptide activate co-cultured skeletal populations in pellets and promote secretion of extracellular matrix collagens and human osteocalcin. Specific temporal release of entrapped RNase A was successfully demonstrated using these specialized microspheres with integrated magnetic beads, which physically disrupted the inorganic macrostructure. These studies demonstrate that bio-inspired calcium carbonate microspheres augment in vivo bone formation in impaction bone grafting. Such microspheres with added biological functionality offer innovative therapeutic approaches to activate skeletal populations and enhance bone formation with reparative implications for hard tissues.

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More information

Published date: April 2009
Keywords: human osteoprogenitor, calcium carbonate, osteogenesis, biomimetic material, mineralization, bone tissue engineering

Identifiers

Local EPrints ID: 151745
URI: http://eprints.soton.ac.uk/id/eprint/151745
ISSN: 0142-9612
PURE UUID: b386038d-5979-462f-b0da-2cb77a6c3c73
ORCID for Janos M. Kanczler: ORCID iD orcid.org/0000-0001-7249-0414
ORCID for Stuart A. Lanham: ORCID iD orcid.org/0000-0002-4516-264X
ORCID for Richard O.C. Oreffo: ORCID iD orcid.org/0000-0001-5995-6726

Catalogue record

Date deposited: 12 May 2010 10:58
Last modified: 14 Mar 2024 02:50

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Contributors

Author: David W. Green
Author: Benjamin J.R. Bolland
Author: Janos M. Kanczler ORCID iD
Author: Stuart A. Lanham ORCID iD
Author: Dominic Walsh
Author: Stephen Mann

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