Augmentation of skeletal tissue formation in impaction bone grafting using vaterite microsphere biocomposites
Augmentation of skeletal tissue formation in impaction bone grafting using vaterite microsphere biocomposites
The development of particulate bone void fillers with added biological function to augment skeletal tissue formation will lead to improved efficacy in bone replacement surgery. We demonstrate the potential for vaterite microsphere biocomposites to augment bone matrix formation within an in vivo model for impaction bone grafting seeded with human bone marrow stromal cells. In vitro tests demonstrate the significance of vaterite microspheres in the activation and promotion of 3D skeletal tissue formation. Further in vitro experiments using functionalized microspheres with surface integrated RGD peptide activate co-cultured skeletal populations in pellets and promote secretion of extracellular matrix collagens and human osteocalcin. Specific temporal release of entrapped RNase A was successfully demonstrated using these specialized microspheres with integrated magnetic beads, which physically disrupted the inorganic macrostructure. These studies demonstrate that bio-inspired calcium carbonate microspheres augment in vivo bone formation in impaction bone grafting. Such microspheres with added biological functionality offer innovative therapeutic approaches to activate skeletal populations and enhance bone formation with reparative implications for hard tissues.
human osteoprogenitor, calcium carbonate, osteogenesis, biomimetic material, mineralization, bone tissue engineering
1918-1927
Green, David W.
9591e846-aeac-4549-b389-1744c5a43cff
Bolland, Benjamin J.R.
1290b975-6eaa-46b8-8dd0-0fb566ad2019
Kanczler, Janos M.
eb8db9ff-a038-475f-9030-48eef2b0559c
Lanham, Stuart A.
28fdbbef-e3b6-4fdf-bd0f-4968eeb614d6
Walsh, Dominic
f5bd569c-0316-4af1-af81-6cc606f10724
Mann, Stephen
1cbb2d4f-aee2-403c-9950-18bd388f02c0
Oreffo, Richard O.C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
April 2009
Green, David W.
9591e846-aeac-4549-b389-1744c5a43cff
Bolland, Benjamin J.R.
1290b975-6eaa-46b8-8dd0-0fb566ad2019
Kanczler, Janos M.
eb8db9ff-a038-475f-9030-48eef2b0559c
Lanham, Stuart A.
28fdbbef-e3b6-4fdf-bd0f-4968eeb614d6
Walsh, Dominic
f5bd569c-0316-4af1-af81-6cc606f10724
Mann, Stephen
1cbb2d4f-aee2-403c-9950-18bd388f02c0
Oreffo, Richard O.C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Green, David W., Bolland, Benjamin J.R., Kanczler, Janos M., Lanham, Stuart A., Walsh, Dominic, Mann, Stephen and Oreffo, Richard O.C.
(2009)
Augmentation of skeletal tissue formation in impaction bone grafting using vaterite microsphere biocomposites.
Biomaterials, 30 (10), .
(doi:10.1016/j.biomaterials.2008.12.052).
Abstract
The development of particulate bone void fillers with added biological function to augment skeletal tissue formation will lead to improved efficacy in bone replacement surgery. We demonstrate the potential for vaterite microsphere biocomposites to augment bone matrix formation within an in vivo model for impaction bone grafting seeded with human bone marrow stromal cells. In vitro tests demonstrate the significance of vaterite microspheres in the activation and promotion of 3D skeletal tissue formation. Further in vitro experiments using functionalized microspheres with surface integrated RGD peptide activate co-cultured skeletal populations in pellets and promote secretion of extracellular matrix collagens and human osteocalcin. Specific temporal release of entrapped RNase A was successfully demonstrated using these specialized microspheres with integrated magnetic beads, which physically disrupted the inorganic macrostructure. These studies demonstrate that bio-inspired calcium carbonate microspheres augment in vivo bone formation in impaction bone grafting. Such microspheres with added biological functionality offer innovative therapeutic approaches to activate skeletal populations and enhance bone formation with reparative implications for hard tissues.
This record has no associated files available for download.
More information
Published date: April 2009
Keywords:
human osteoprogenitor, calcium carbonate, osteogenesis, biomimetic material, mineralization, bone tissue engineering
Identifiers
Local EPrints ID: 151745
URI: http://eprints.soton.ac.uk/id/eprint/151745
ISSN: 0142-9612
PURE UUID: b386038d-5979-462f-b0da-2cb77a6c3c73
Catalogue record
Date deposited: 12 May 2010 10:58
Last modified: 14 Mar 2024 02:50
Export record
Altmetrics
Contributors
Author:
David W. Green
Author:
Benjamin J.R. Bolland
Author:
Janos M. Kanczler
Author:
Stuart A. Lanham
Author:
Dominic Walsh
Author:
Stephen Mann
Download statistics
Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.
View more statistics