Biocompatibility and osteogenic potential of human fetal femur-derived cells on surface selective laser sintered scaffolds
Biocompatibility and osteogenic potential of human fetal femur-derived cells on surface selective laser sintered scaffolds
For optimal bone regeneration, scaffolds need to fit anatomically into the requisite bone defects and, ideally, augment cell growth and differentiation. In this study we evaluated novel computationally designed surface selective laser sintering (SSLS) scaffolds for their biocompatibility as templates, in vitro and in vivo, for human fetal femur-derived cell viability, growth and osteogenesis. Fetal femur-derived cells were successfully cultured on SSLS-poly(d,l)-lactic acid (SSLS-PLA) scaffolds expressing alkaline phosphatase activity after 7 days. Cell proliferation, ingrowth, Alcian blue/Sirius red and type I collagen positive staining of matrix deposition were observed for fetal femur-derived cells cultured on SSLS-PLA scaffolds in vitro and in vivo. SSLS-PLA scaffolds and SSLS-PLA scaffolds seeded with fetal femur-derived cells implanted into a murine critical-sized femur segmental defect model aided the regeneration of the bone defect. SSLS techniques allow fabrication of biocompatible/biodegradable scaffolds, computationally designed to fit any defect, providing a template for cell osteogenesis in vitro and in vivo.
tissue engineering, surface selective laser sintering, human fetal femur-derived cells, osteogenesis
2063-2071
Kanczler, Janos M.
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Mirmalek-Sani, Sayed-Hadi
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Hanley, Neil A.
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Ivanov, Alexander L.
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Barry, John J.A.
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Upton, Claire
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Shakesheff, Kevin M.
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Howdle, Steven M.
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Antonov, Eugeuni N.
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Bagratashvili, Victor N.
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Popov, Vladimir K.
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Oreffo, Richard O.C.
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July 2009
Kanczler, Janos M.
eb8db9ff-a038-475f-9030-48eef2b0559c
Mirmalek-Sani, Sayed-Hadi
af9188b9-3124-40c0-8cd4-203a0fb490f9
Hanley, Neil A.
bf03f7bb-f377-44fb-8344-0bb1ca8b2ef9
Ivanov, Alexander L.
cc5fd2d4-55ae-43ec-9316-b253a48da576
Barry, John J.A.
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Upton, Claire
e3b6e1ab-dc42-49d5-895d-9c9708d3628c
Shakesheff, Kevin M.
5b9ed879-e2c5-4c62-bb38-abd2bb4960ac
Howdle, Steven M.
ec70f53e-a5df-4e99-9da2-f90582dde80b
Antonov, Eugeuni N.
b278be65-9fe0-4cea-ac70-f1097796e559
Bagratashvili, Victor N.
7856eec5-fc1a-445e-95a8-cd8d9c0683d1
Popov, Vladimir K.
65bf7481-bd43-443a-a7c3-0a3936538fda
Oreffo, Richard O.C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Kanczler, Janos M., Mirmalek-Sani, Sayed-Hadi, Hanley, Neil A., Ivanov, Alexander L., Barry, John J.A., Upton, Claire, Shakesheff, Kevin M., Howdle, Steven M., Antonov, Eugeuni N., Bagratashvili, Victor N., Popov, Vladimir K. and Oreffo, Richard O.C.
(2009)
Biocompatibility and osteogenic potential of human fetal femur-derived cells on surface selective laser sintered scaffolds.
Acta Biomaterialia, 5 (6), .
(doi:10.1016/j.actbio.2009.03.010).
Abstract
For optimal bone regeneration, scaffolds need to fit anatomically into the requisite bone defects and, ideally, augment cell growth and differentiation. In this study we evaluated novel computationally designed surface selective laser sintering (SSLS) scaffolds for their biocompatibility as templates, in vitro and in vivo, for human fetal femur-derived cell viability, growth and osteogenesis. Fetal femur-derived cells were successfully cultured on SSLS-poly(d,l)-lactic acid (SSLS-PLA) scaffolds expressing alkaline phosphatase activity after 7 days. Cell proliferation, ingrowth, Alcian blue/Sirius red and type I collagen positive staining of matrix deposition were observed for fetal femur-derived cells cultured on SSLS-PLA scaffolds in vitro and in vivo. SSLS-PLA scaffolds and SSLS-PLA scaffolds seeded with fetal femur-derived cells implanted into a murine critical-sized femur segmental defect model aided the regeneration of the bone defect. SSLS techniques allow fabrication of biocompatible/biodegradable scaffolds, computationally designed to fit any defect, providing a template for cell osteogenesis in vitro and in vivo.
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Published date: July 2009
Keywords:
tissue engineering, surface selective laser sintering, human fetal femur-derived cells, osteogenesis
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Local EPrints ID: 151761
URI: http://eprints.soton.ac.uk/id/eprint/151761
ISSN: 1742-7061
PURE UUID: 416f1e96-2491-453f-bd70-87410b3f2b1f
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Date deposited: 12 May 2010 11:11
Last modified: 14 Mar 2024 02:50
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Contributors
Author:
Janos M. Kanczler
Author:
Sayed-Hadi Mirmalek-Sani
Author:
Neil A. Hanley
Author:
Alexander L. Ivanov
Author:
John J.A. Barry
Author:
Claire Upton
Author:
Kevin M. Shakesheff
Author:
Steven M. Howdle
Author:
Eugeuni N. Antonov
Author:
Victor N. Bagratashvili
Author:
Vladimir K. Popov
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