Incorporation of cis-9, trans-11 or trans-10, cis-12 conjugated linoleic acid into plasma and cellular lipids in healthy men

Description/Abstract

This study investigated the incorporation of
cis
-
9,
trans
-11 conjugated linoleic acid (c9,t11 CLA) and
trans
-
10,
cis
-12-CLA (t10,c12 CLA) into plasma and peripheral
blood mononuclear cell (PBMC) lipids when consumed as
supplements highly enriched in these isomers. Healthy
men (n
�
49, age 31
�
8 years) consumed one, two, and
four capsules containing
�
600 mg of either c9,t11 CLA or
t10,c12 CLA per capsule for sequential 8 week periods followed
by a 6 week washout before consuming the alternative
isomer. Both isomers were incorporated in a dosedependent
manner into plasma phosphatidylcholine (PC)
(c9,t11 CLA
r
�
0.779, t10,c12 CLA
r
�
0.738;
P
�
0.0001)
and cholesteryl ester (CE) (c9,t11 CLA
r
�
0.706, t10,c12
CLA
r
�
0.788;
P
�
0.0001). Only t10,c12 CLA was enriched
in plasma nonesterified fatty acids. Both c9,t11 CLA
and t10,c12 CLA were incorporated linearly into PBMC total
lipids (
r
�
0.285 and
r
�
0.273, respectively;
P
�
0.0005). The highest concentrations of c9,t11 CLA and
t10,c12 CLA in PBMC lipids were 3- to 4-fold lower than
those in plasma PC and CE. These data suggest that the
level of intake is a major determinant of plasma and PBMC
CLA content, although PBMCs appear to incorporate both
CLA isomers less readily.—
Burdge, G. C., B. Lupoli, J. J.
Russell, S. Tricon, S. Kew, T. Banerjee, K. J. Shingfield, D. E.
Beever, R. F. Grimble, C. M. Williams, P. Yaqoob, and P. C.
Calder.
Incorporation of
cis
-9,
trans
-11 or
trans
-10,
cis
-12 conjugated
linoleic acid into plasma and cellular lipids in
healthy men.