Fish oil and antioxidants alter the composition and function of circulating mononuclear cells in Crohn disease
Fish oil and antioxidants alter the composition and function of circulating mononuclear cells in Crohn disease
Background: Crohn disease (CD) is associated with osteoporosis and other extraintestinal manifestations that might be mediated by cytokines from circulating (peripheral blood) mononuclear cells (PBMCs). Fish oil rich in eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) reduces disease activity in patients with CD with raised laboratory markers of inflammation and in healthy subjects alters PBMC function.
Objective: We investigated the effect of fish oil plus antioxidants on cytokine production by PBMCs from patients with CD with raised C-reactive protein concentrations (?6.9 mg/L) or erythrocyte sedimentation rates (?18 mm/h).
Design: A randomized placebo-controlled trial of fish oil (2.7 g EPA and DHA/d; n = 31) or placebo (olive oil; n = 31) for 24 wk was conducted in patients with CD. The fish-oil group additionally received an antioxidant preparation (vitamins A, C, and E and selenium). Exclusion criteria included corticosteroid use. Fatty acid composition was measured by gas chromatography. Production of tumor necrosis factor ?, interferon ? (IFN-?), and prostaglandin E2 (PGE2) was measured by enzyme-linked immunosorbent assays after stimulation with mitogen and endotoxin (lipopolysaccharide).
Results: Fish-oil plus antioxidant dietary supplementation was associated with higher EPA and DHA incorporation into PBMCs (P < 0.001) and lower arachidonic acid (P = 0.006) and lower production of IFN-? by mitogen-stimulated PBMCs (P = 0.012) and of PGE2 by lipopolysaccharide-stimulated PBMCs (P = 0.047).
Conclusion: Dietary supplementation with fish oil plus antioxidants is associated with modified PBMC composition and lower production of PGE2 and IFN-? by circulating monocytes or macrophages. The response of extraintestinal manifestations of CD should be investigated in a randomized controlled trial.
mononuclear cell, cytokines, polyunsaturated fatty acids, crohn disease
1137-1144
Trebble, Timothy M.
48119242-d090-4778-afd9-58f451dbeecf
Arden, Nigel K.
23af958d-835c-4d79-be54-4bbe4c68077f
Wootton, Stephen A.
bf47ef35-0b33-4edb-a2b0-ceda5c475c0c
Calder, Philip C.
1797e54f-378e-4dcb-80a4-3e30018f07a6
Mullee, Mark A.
fd3f91c3-5e95-4f56-8d73-260824eeb362
Fine, David R.
ce3d6a77-040e-4aec-a8f5-4c4c22431605
Stroud, Mike A.
1665ae65-0898-4848-bf0d-baec8f2bb078
November 2004
Trebble, Timothy M.
48119242-d090-4778-afd9-58f451dbeecf
Arden, Nigel K.
23af958d-835c-4d79-be54-4bbe4c68077f
Wootton, Stephen A.
bf47ef35-0b33-4edb-a2b0-ceda5c475c0c
Calder, Philip C.
1797e54f-378e-4dcb-80a4-3e30018f07a6
Mullee, Mark A.
fd3f91c3-5e95-4f56-8d73-260824eeb362
Fine, David R.
ce3d6a77-040e-4aec-a8f5-4c4c22431605
Stroud, Mike A.
1665ae65-0898-4848-bf0d-baec8f2bb078
Trebble, Timothy M., Arden, Nigel K., Wootton, Stephen A., Calder, Philip C., Mullee, Mark A., Fine, David R. and Stroud, Mike A.
(2004)
Fish oil and antioxidants alter the composition and function of circulating mononuclear cells in Crohn disease.
American Journal of Clinical Nutrition, 80 (5), .
Abstract
Background: Crohn disease (CD) is associated with osteoporosis and other extraintestinal manifestations that might be mediated by cytokines from circulating (peripheral blood) mononuclear cells (PBMCs). Fish oil rich in eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) reduces disease activity in patients with CD with raised laboratory markers of inflammation and in healthy subjects alters PBMC function.
Objective: We investigated the effect of fish oil plus antioxidants on cytokine production by PBMCs from patients with CD with raised C-reactive protein concentrations (?6.9 mg/L) or erythrocyte sedimentation rates (?18 mm/h).
Design: A randomized placebo-controlled trial of fish oil (2.7 g EPA and DHA/d; n = 31) or placebo (olive oil; n = 31) for 24 wk was conducted in patients with CD. The fish-oil group additionally received an antioxidant preparation (vitamins A, C, and E and selenium). Exclusion criteria included corticosteroid use. Fatty acid composition was measured by gas chromatography. Production of tumor necrosis factor ?, interferon ? (IFN-?), and prostaglandin E2 (PGE2) was measured by enzyme-linked immunosorbent assays after stimulation with mitogen and endotoxin (lipopolysaccharide).
Results: Fish-oil plus antioxidant dietary supplementation was associated with higher EPA and DHA incorporation into PBMCs (P < 0.001) and lower arachidonic acid (P = 0.006) and lower production of IFN-? by mitogen-stimulated PBMCs (P = 0.012) and of PGE2 by lipopolysaccharide-stimulated PBMCs (P = 0.047).
Conclusion: Dietary supplementation with fish oil plus antioxidants is associated with modified PBMC composition and lower production of PGE2 and IFN-? by circulating monocytes or macrophages. The response of extraintestinal manifestations of CD should be investigated in a randomized controlled trial.
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Published date: November 2004
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Original research communication
Keywords:
mononuclear cell, cytokines, polyunsaturated fatty acids, crohn disease
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Local EPrints ID: 152637
URI: http://eprints.soton.ac.uk/id/eprint/152637
ISSN: 0002-9165
PURE UUID: 04328c51-0391-4da8-a911-d6b12da6f17d
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Date deposited: 03 Jun 2011 14:37
Last modified: 14 Mar 2024 02:39
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Author:
Timothy M. Trebble
Author:
David R. Fine
Author:
Mike A. Stroud
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