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Ratio of n-6 to n-3 fatty acids in the diet arrects tumor growth and cachexia in Walker 256 tumor-bearing rats

Ratio of n-6 to n-3 fatty acids in the diet arrects tumor growth and cachexia in Walker 256 tumor-bearing rats
Ratio of n-6 to n-3 fatty acids in the diet arrects tumor growth and cachexia in Walker 256 tumor-bearing rats
In this study we investigate the impact of the dietary ratio of n-6 to n-3 fatty acids (FAs) from postweaning until adult age upon tumor growth, lipid peroxidation in tumor tissue, and metabolic indicators of cancer cachexia in Walker 256 tumor-bearing rats. Weanling male Wistar rats received a normal low-fat (40 g/kg diet) chow diet or high-fat diets (300 g/kg) that included fish oil (FO) or sunflower oil or blends of FO and sunflower oil to yield n-6 to n-3 FA ratios of approximately 6:1, 30:1, and 60:1 ad libitum. After 8 wk, half of each group was inoculated with 1 ml of 2 x 10(7) Walker 256 cells. At the 14th day after tumor inoculation, the animals were killed, and tumors and blood were removed. The different diets did not modify the blood parameters in the absence of tumor bearing, except the high-FO diet, which decreased serum cholesterol and triacylglycerol concentrations. Tumor weight in chow-fed rats was 19 g, and these rats displayed cancer cachexia, characterized by hypoglycemia, hyperlacticidemia, hypertriacylglycerolemia, loss of body weight, and food intake reduction. Tumor weight in FO-fed rats was 7.7 g, and these animals gained body weight (14.6 g) and maintained blood metabolic parameters similar to non-tumor-bearing animals. Tumor weight in rats fed the diet with an n-6 to n-3 FA ratio of 6:1 was similar to tumor-bearing, chow-fed rats, but they gained 2 g in the body weight and blood metabolic parameters were similar to those in non-tumor-bearing rats. However, a further increase in the n-6 FA content of the diet did not change the cachectic state associated with tumor bearing. In this experimental model, a dietary n-6 to n-3 FA ratio of 6:1 was able to increase food intake and body weight, restore the biochemical blood parameters of cachexia, and prevent the development of cancer cachexia.

0163-5581
194-201
Pizato, Nathalia
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Bonatto, Sandro
b6deac87-744a-4bdc-aa63-c739ad06cf20
Yamazaki, Ricardo K.
dc3167fd-8a53-4bc1-b099-113334382bd1
Aikawa, Julia
87615dc7-5d4f-4e2c-a605-97b92dc4cf1f
Nogata, Claudia
0f592a36-61da-4197-91ba-98351b0632c7
Mund, Rogeria C.
89c26a6c-cdd8-4e5a-b5d5-40fefffcfcc8
Nunes, Everson A.
62a7f5db-40d7-4814-a1d0-2c1eb71a9c29
Piconcelli, Maressa
afb0a1eb-39ae-44d8-a852-18c5b1a56a31
Naliwaiko, Katya
6be7df1b-6612-43c9-849f-d1e760337d9d
Curi, Rui
87abee54-a75d-4e94-b9b9-2169d8f97fec
Calder, Philip C.
1797e54f-378e-4dcb-80a4-3e30018f07a6
Fernandes, Luiz Claudio
aca8e685-96a8-4aee-b941-53987cdc82c4
Pizato, Nathalia
506fb966-a881-4a09-a467-2df7b07995c5
Bonatto, Sandro
b6deac87-744a-4bdc-aa63-c739ad06cf20
Yamazaki, Ricardo K.
dc3167fd-8a53-4bc1-b099-113334382bd1
Aikawa, Julia
87615dc7-5d4f-4e2c-a605-97b92dc4cf1f
Nogata, Claudia
0f592a36-61da-4197-91ba-98351b0632c7
Mund, Rogeria C.
89c26a6c-cdd8-4e5a-b5d5-40fefffcfcc8
Nunes, Everson A.
62a7f5db-40d7-4814-a1d0-2c1eb71a9c29
Piconcelli, Maressa
afb0a1eb-39ae-44d8-a852-18c5b1a56a31
Naliwaiko, Katya
6be7df1b-6612-43c9-849f-d1e760337d9d
Curi, Rui
87abee54-a75d-4e94-b9b9-2169d8f97fec
Calder, Philip C.
1797e54f-378e-4dcb-80a4-3e30018f07a6
Fernandes, Luiz Claudio
aca8e685-96a8-4aee-b941-53987cdc82c4

Pizato, Nathalia, Bonatto, Sandro, Yamazaki, Ricardo K., Aikawa, Julia, Nogata, Claudia, Mund, Rogeria C., Nunes, Everson A., Piconcelli, Maressa, Naliwaiko, Katya, Curi, Rui, Calder, Philip C. and Fernandes, Luiz Claudio (2005) Ratio of n-6 to n-3 fatty acids in the diet arrects tumor growth and cachexia in Walker 256 tumor-bearing rats. Nutrition and Cancer, 53 (2), 194-201. (doi:10.1207/s15327914nc5302_8).

Record type: Article

Abstract

In this study we investigate the impact of the dietary ratio of n-6 to n-3 fatty acids (FAs) from postweaning until adult age upon tumor growth, lipid peroxidation in tumor tissue, and metabolic indicators of cancer cachexia in Walker 256 tumor-bearing rats. Weanling male Wistar rats received a normal low-fat (40 g/kg diet) chow diet or high-fat diets (300 g/kg) that included fish oil (FO) or sunflower oil or blends of FO and sunflower oil to yield n-6 to n-3 FA ratios of approximately 6:1, 30:1, and 60:1 ad libitum. After 8 wk, half of each group was inoculated with 1 ml of 2 x 10(7) Walker 256 cells. At the 14th day after tumor inoculation, the animals were killed, and tumors and blood were removed. The different diets did not modify the blood parameters in the absence of tumor bearing, except the high-FO diet, which decreased serum cholesterol and triacylglycerol concentrations. Tumor weight in chow-fed rats was 19 g, and these rats displayed cancer cachexia, characterized by hypoglycemia, hyperlacticidemia, hypertriacylglycerolemia, loss of body weight, and food intake reduction. Tumor weight in FO-fed rats was 7.7 g, and these animals gained body weight (14.6 g) and maintained blood metabolic parameters similar to non-tumor-bearing animals. Tumor weight in rats fed the diet with an n-6 to n-3 FA ratio of 6:1 was similar to tumor-bearing, chow-fed rats, but they gained 2 g in the body weight and blood metabolic parameters were similar to those in non-tumor-bearing rats. However, a further increase in the n-6 FA content of the diet did not change the cachectic state associated with tumor bearing. In this experimental model, a dietary n-6 to n-3 FA ratio of 6:1 was able to increase food intake and body weight, restore the biochemical blood parameters of cachexia, and prevent the development of cancer cachexia.

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Published date: 2005

Identifiers

Local EPrints ID: 152829
URI: http://eprints.soton.ac.uk/id/eprint/152829
ISSN: 0163-5581
PURE UUID: 81d21a23-4fd3-4816-b95d-36744ef94a82
ORCID for Philip C. Calder: ORCID iD orcid.org/0000-0002-6038-710X

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Date deposited: 28 Jun 2010 08:50
Last modified: 14 Mar 2024 02:39

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Contributors

Author: Nathalia Pizato
Author: Sandro Bonatto
Author: Ricardo K. Yamazaki
Author: Julia Aikawa
Author: Claudia Nogata
Author: Rogeria C. Mund
Author: Everson A. Nunes
Author: Maressa Piconcelli
Author: Katya Naliwaiko
Author: Rui Curi
Author: Luiz Claudio Fernandes

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