High-speed particle detection in a micro-Coulter counter with two-dimensional adjustable aperture
Rodriguez-Trujillo, Romen, Castillo-Fernandez, Oscar, Garrido, Miquel, Arundell, Martin, Valencia, Antoni and Gomila, Gabriel (2008) High-speed particle detection in a micro-Coulter counter with two-dimensional adjustable aperture. Biosensors and Bioelectronics (doi:10.1016/j.bios.2008.04.005).
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This article presents the fabrication and characterisation of a high-speed detection micro-Coulter counter with two-dimensional (2D) adjustable aperture and differential impedance detection. The developed device has been fabricated from biocompatible and transparent materials (polymer and glass) and uses the principle of hydrodynamic focusing in two dimensions. The use of a conductive solution for the sample flux and non-conductive solutions for the focalising fluxes provides an adjustable sample flow where particles are aligned and the resistive response concentrated, consequently enhancing the sensitivity and versatility of the device. High-speed counting of 20 μm polystyrene particles and 5 μm yeast cells with a rate of up to 1000 particles/s has been demonstrated. Two-dimensional focusing conditions have been used in devices with physical cross-sectional areas of 180 μm × 65 μm and 100 μm × 43 μm, respectively, in which particles resulted undetectable in the absence of focusing. The 2D-focusing conditions have provided, in addition, increased detection sensitivity by a factor of 1.6 as compared to 1D-focusing conditions
|Digital Object Identifier (DOI):||doi:10.1016/j.bios.2008.04.005|
|Keywords:||microfluidics, poly-dimethylsiloxane, microcytometer, impedance, microelectrodes, soft lithography|
|Subjects:||Q Science > Q Science (General)|
|Divisions:||University Structure - Pre August 2011 > School of Biological Sciences
|Date Deposited:||28 May 2010 14:11|
|Last Modified:||06 Aug 2015 03:01|
|RDF:||RDF+N-Triples, RDF+N3, RDF+XML, Browse.|
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