Acylation of streptomyces type II polyketide synthase acyl carrier proteins


Crosby, John, Byrom, Kate J. , Hitchman, Timothy S. , Cox, Russell J. , Crump, Matthew P. , Findlow, I. Stuart C. , Bibb, Maureen J. and Simpson, Thomas J. (1998) Acylation of streptomyces type II polyketide synthase acyl carrier proteins. FEBS Letters, 433, (1-2), 132-138. (doi:10.1016/S0014-5793(98)00840-0).

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Description/Abstract

Acyl derivatives of type II PKS ACPs are required for in vitro studies of polyketide biosynthesis. The presence of an exposed cysteine residue prevented specific chemical acylation of the phosphopantetheine thiol of the actinorhodin PKS holo ACP. Acylation studies were further complicated by intramolecular disulphide formation between cysteine 17 and the phosphopantetheine. The presence of this intramolecular disulphide was confirmed by tryptic digestion of the ACP followed by ESMS analysis of the fragments. An act Cys17Ser ACP was engineered by site-directed mutagenesis. S-Acyl adducts of act C17S, oxytetracycline and griseusin holo ACPs were rapidly formed by reaction with hexanoyl, 5-ketohexanoyl and protected acetoacetyl imidazolides. Comparisons with type II FAS ACPs were made.

Item Type: Article
ISSNs: 0014-5793 (print)
1873-3468 (electronic)
Related URLs:
Subjects: Q Science > Q Science (General)
Divisions: University Structure - Pre August 2011 > School of Biological Sciences
ePrint ID: 156641
Date Deposited: 01 Jun 2010 12:21
Last Modified: 27 Mar 2014 19:14
URI: http://eprints.soton.ac.uk/id/eprint/156641

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