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Development of a laboratory model to assess the removal of biofilm from interproximal spaces by powered tooth brushing

Development of a laboratory model to assess the removal of biofilm from interproximal spaces by powered tooth brushing
Development of a laboratory model to assess the removal of biofilm from interproximal spaces by powered tooth brushing
PURPOSE: To develop an interproximal laboratory model to compare the potential effectiveness of powered brushing to remove biofilm plaque from interproximal spaces beyond the reach of bristles. MATERIALS AND METHODS: Streptococcus mutans biofilms were first grown on glass microscope slides in a drip-flow reactor. The slides were removed and positioned in the interproximal model. Each slide was exposed to 15 seconds powered brushing with either the Sonicare Elite or the Braun Oral-B 3D Excel. The thickness of the biofilm was measured with confocal microscopy at various distances from the bristle tips. RESULTS: The Sonicare Elite reduced the thickness of biofilm by 57% at a distance of 0-5 mm from the bristle tips, 53% at 5-10 mm and 43% at 10-15 mm, relative to biofilm in areas unexposed to brushing. All reductions in thickness were statistically significant (P < 0.01). The Braun Oral-B 3D Excel reduced the biofilm thickness by 16%, 13%, and 19% at the same distances respectively, but the thickness reductions were not statistically significant from those in the unexposed areas (P > 0.1).
0894-8275
12B-17B
Adams, Heather
85771f33-0f4d-49c7-af59-41b080efd4bd
Winston, Matthew T.
49d95176-67cf-4f6c-b91d-85ef97b9191a
Heersink, Joanna
8f115aac-87fd-4d8d-9849-500c7f311efd
Buckingham-Meyer, Kelli A.
4a93fdbe-b766-45e6-b011-c42d5bc6270f
Costerton, J. William
3561239b-c96e-41af-9228-4fc120466c4b
Stoodley, Paul
08614665-92a9-4466-806e-20c6daeb483f
Adams, Heather
85771f33-0f4d-49c7-af59-41b080efd4bd
Winston, Matthew T.
49d95176-67cf-4f6c-b91d-85ef97b9191a
Heersink, Joanna
8f115aac-87fd-4d8d-9849-500c7f311efd
Buckingham-Meyer, Kelli A.
4a93fdbe-b766-45e6-b011-c42d5bc6270f
Costerton, J. William
3561239b-c96e-41af-9228-4fc120466c4b
Stoodley, Paul
08614665-92a9-4466-806e-20c6daeb483f

Adams, Heather, Winston, Matthew T., Heersink, Joanna, Buckingham-Meyer, Kelli A., Costerton, J. William and Stoodley, Paul (2002) Development of a laboratory model to assess the removal of biofilm from interproximal spaces by powered tooth brushing. [in special issue: Special Issue] American Journal of Dentistry, 15, 12B-17B.

Record type: Article

Abstract

PURPOSE: To develop an interproximal laboratory model to compare the potential effectiveness of powered brushing to remove biofilm plaque from interproximal spaces beyond the reach of bristles. MATERIALS AND METHODS: Streptococcus mutans biofilms were first grown on glass microscope slides in a drip-flow reactor. The slides were removed and positioned in the interproximal model. Each slide was exposed to 15 seconds powered brushing with either the Sonicare Elite or the Braun Oral-B 3D Excel. The thickness of the biofilm was measured with confocal microscopy at various distances from the bristle tips. RESULTS: The Sonicare Elite reduced the thickness of biofilm by 57% at a distance of 0-5 mm from the bristle tips, 53% at 5-10 mm and 43% at 10-15 mm, relative to biofilm in areas unexposed to brushing. All reductions in thickness were statistically significant (P < 0.01). The Braun Oral-B 3D Excel reduced the biofilm thickness by 16%, 13%, and 19% at the same distances respectively, but the thickness reductions were not statistically significant from those in the unexposed areas (P > 0.1).

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More information

Published date: November 2002
Organisations: Engineering Mats & Surface Engineerg Gp

Identifiers

Local EPrints ID: 157131
URI: http://eprints.soton.ac.uk/id/eprint/157131
ISSN: 0894-8275
PURE UUID: 3e237761-2ffe-4df3-874c-2ec0947c2847
ORCID for Paul Stoodley: ORCID iD orcid.org/0000-0001-6069-273X

Catalogue record

Date deposited: 09 Jun 2010 14:27
Last modified: 09 Jan 2022 03:32

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Contributors

Author: Heather Adams
Author: Matthew T. Winston
Author: Joanna Heersink
Author: Kelli A. Buckingham-Meyer
Author: J. William Costerton
Author: Paul Stoodley ORCID iD

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