Polarised light microscopy of human amniotic fluid: a rapid method to assess fetal lung maturity - a pilot study
Clark, H.W. and Jacobson, W. (1991) Polarised light microscopy of human amniotic fluid: a rapid method to assess fetal lung maturity - a pilot study. Journal of Obstetrics and Gynaecology, 11, (5), 307-314.
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Particles of pulmonary surfactant can be detected in
minute quantities of amniotic fluid; they appear as round
birefringent particles of 0.5-3 pm diameter with a characteristic cross of polarisation. Two hundred and five samples of amniotic fluid of various gestational ages were examined microscopically under polarised light. The number of birefringent particles observed per pI of amniotic fluid correlated well with the percentage of phosphatidyl-choline in the sample. There was also good agreement with palmitate determinations, surface tension measurements. the
1ecithin:sphingomyelin ratio and the clinical data. This technique is simple and rapid and does not require counting surfactant particles.
|Subjects:||R Medicine > R Medicine (General)
R Medicine > RG Gynecology and obstetrics
R Medicine > RJ Pediatrics
|Divisions:||University Structure - Pre August 2011 > School of Medicine
|Date Deposited:||24 Jun 2010 09:28|
|Last Modified:||02 Mar 2012 11:35|
|RDF:||RDF+N-Triples, RDF+N3, RDF+XML, Browse.|
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