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Holographically encoded microparticles for bead-based assays

Holographically encoded microparticles for bead-based assays
Holographically encoded microparticles for bead-based assays
We demonstrate a re-writable, high capacity holographic encoding technique for multiplexed bead-based suspension assays. The microparticles are made from SU8 doped with a photochromic diarylethene dye and manufactured using multilayer photolithography and dry etching. Each particle is encoded with a unique hologram, whose diffraction pattern consists of bright and dark regions, representing a binary number that identifies the particle. Theoretically up to 1024 unique codes are available on a 100 µm particle using this method, when the code is read with a standard 2/3" CMOS camera. Encoding capacities of 512 unique codes have been demonstrated on a 500 µm SU8 particle. The code is thermally stable for 3 days at 25 °C, and once written, the code can be erased and re-written once whilst still remaining readable. The code can be written into the particle during an assay experiment (no pre-encoding is required) and requires simple optics for reading.
0022-3727
55507
Birtwell, Sam W.
40be7fb5-3cc9-4a4a-8e8f-97e13a0d9b40
Banu, Shahanara
5e1caab9-665e-4a17-b582-28a059acdf06
Zheludev, Nikolay I.
32fb6af7-97e4-4d11-bca6-805745e40cc6
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Birtwell, Sam W.
40be7fb5-3cc9-4a4a-8e8f-97e13a0d9b40
Banu, Shahanara
5e1caab9-665e-4a17-b582-28a059acdf06
Zheludev, Nikolay I.
32fb6af7-97e4-4d11-bca6-805745e40cc6
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174

Birtwell, Sam W., Banu, Shahanara, Zheludev, Nikolay I. and Morgan, Hywel (2009) Holographically encoded microparticles for bead-based assays. Journal of Physics D: Applied Physics, 42 (5), 55507. (doi:10.1088/0022-3727/42/5/055507).

Record type: Article

Abstract

We demonstrate a re-writable, high capacity holographic encoding technique for multiplexed bead-based suspension assays. The microparticles are made from SU8 doped with a photochromic diarylethene dye and manufactured using multilayer photolithography and dry etching. Each particle is encoded with a unique hologram, whose diffraction pattern consists of bright and dark regions, representing a binary number that identifies the particle. Theoretically up to 1024 unique codes are available on a 100 µm particle using this method, when the code is read with a standard 2/3" CMOS camera. Encoding capacities of 512 unique codes have been demonstrated on a 500 µm SU8 particle. The code is thermally stable for 3 days at 25 °C, and once written, the code can be erased and re-written once whilst still remaining readable. The code can be written into the particle during an assay experiment (no pre-encoding is required) and requires simple optics for reading.

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More information

Published date: 19 February 2009
Organisations: Optoelectronics Research Centre, Electronics & Computer Science

Identifiers

Local EPrints ID: 163441
URI: http://eprints.soton.ac.uk/id/eprint/163441
ISSN: 0022-3727
PURE UUID: 1985095d-2d3d-4eaf-b6a2-f76940f142ea
ORCID for Nikolay I. Zheludev: ORCID iD orcid.org/0000-0002-1013-6636
ORCID for Hywel Morgan: ORCID iD orcid.org/0000-0003-4850-5676

Catalogue record

Date deposited: 08 Sep 2010 13:05
Last modified: 14 Mar 2024 02:48

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Contributors

Author: Sam W. Birtwell
Author: Shahanara Banu
Author: Nikolay I. Zheludev ORCID iD
Author: Hywel Morgan ORCID iD

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