The University of Southampton
University of Southampton Institutional Repository

Role for CED-9 and Egl-1 as regulators of mitochondrial fission and fusion dynamics

Role for CED-9 and Egl-1 as regulators of mitochondrial fission and fusion dynamics
Role for CED-9 and Egl-1 as regulators of mitochondrial fission and fusion dynamics
Bcl-2 family proteins play central roles in apoptosis by regulating the release of mitochondrial intermembrane space proteins such as cytochrome c. Death-promoting Bcl-2 family members, such as Bax, can promote cytochrome c release and fragmentation of the mitochondrial network, whereas apoptosis-inhibitory members, such as Bcl-2 and Bcl-xL, can antagonize these events. It remains unclear whether CED-9, the worm Bcl-2 relative, can regulate mitochondrial fission/fusion dynamics or the release of proteins from the mitochondrial intermembrane space. Here, we show that CED-9 interacts with Mitofusin-2/fuzzy onions and can promote mitochondrial clustering and dramatic reorganization of mitochondrial networks. Consistent with its ability to neutralize CED-9 function, EGL-1 antagonized CED-9-dependent remodeling of the mitochondrial network. However, CED-9 failed to inhibit mitochondrial cytochrome c release or apoptosis induced by diverse triggers in mammalian cells. These data suggest that the ability to regulate mitochondrial fission/fusion dynamics is an evolutionarily conserved property of the Bcl-2 family.
1097-2765
761-773
Delivani, Petrina
8840fdf6-c287-4dbf-837c-56a36b1e7e0e
Adrain, Colin
f7950ceb-b788-4d86-a607-b9efc4437142
Taylor, Rebecca C.
72e4edb9-e8bb-47ad-a544-145a50896d23
Duriez, Patrick J.
4cf499bc-007a-43b3-b180-d6e5dc3d151b
Martin, Seamus J.
3df02649-5634-44da-834d-098937a7ae5b
Delivani, Petrina
8840fdf6-c287-4dbf-837c-56a36b1e7e0e
Adrain, Colin
f7950ceb-b788-4d86-a607-b9efc4437142
Taylor, Rebecca C.
72e4edb9-e8bb-47ad-a544-145a50896d23
Duriez, Patrick J.
4cf499bc-007a-43b3-b180-d6e5dc3d151b
Martin, Seamus J.
3df02649-5634-44da-834d-098937a7ae5b

Delivani, Petrina, Adrain, Colin, Taylor, Rebecca C., Duriez, Patrick J. and Martin, Seamus J. (2006) Role for CED-9 and Egl-1 as regulators of mitochondrial fission and fusion dynamics. Molecular Cell, 21 (6), 761-773. (doi:10.1016/j.molcel.2006.01.034). (PMID:16543141)

Record type: Article

Abstract

Bcl-2 family proteins play central roles in apoptosis by regulating the release of mitochondrial intermembrane space proteins such as cytochrome c. Death-promoting Bcl-2 family members, such as Bax, can promote cytochrome c release and fragmentation of the mitochondrial network, whereas apoptosis-inhibitory members, such as Bcl-2 and Bcl-xL, can antagonize these events. It remains unclear whether CED-9, the worm Bcl-2 relative, can regulate mitochondrial fission/fusion dynamics or the release of proteins from the mitochondrial intermembrane space. Here, we show that CED-9 interacts with Mitofusin-2/fuzzy onions and can promote mitochondrial clustering and dramatic reorganization of mitochondrial networks. Consistent with its ability to neutralize CED-9 function, EGL-1 antagonized CED-9-dependent remodeling of the mitochondrial network. However, CED-9 failed to inhibit mitochondrial cytochrome c release or apoptosis induced by diverse triggers in mammalian cells. These data suggest that the ability to regulate mitochondrial fission/fusion dynamics is an evolutionarily conserved property of the Bcl-2 family.

This record has no associated files available for download.

More information

Published date: 17 March 2006

Identifiers

Local EPrints ID: 164799
URI: http://eprints.soton.ac.uk/id/eprint/164799
ISSN: 1097-2765
PURE UUID: c64ec1f6-a2ba-4769-b1bb-538addb1aad3
ORCID for Patrick J. Duriez: ORCID iD orcid.org/0000-0003-1814-2552

Catalogue record

Date deposited: 04 Oct 2010 14:48
Last modified: 14 Mar 2024 02:52

Export record

Altmetrics

Contributors

Author: Petrina Delivani
Author: Colin Adrain
Author: Rebecca C. Taylor
Author: Patrick J. Duriez ORCID iD
Author: Seamus J. Martin

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×