Honey promotes angiogenic activity in the rat aortic ring assay

Rossiter, K., Cooper, A.J., Voegeli, D. and Lwaleed, B.A. (2010) Honey promotes angiogenic activity in the rat aortic ring assay. Journal of Wound Care, 19, (10), 440-446.


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Objective: To investigate possible effects of honey on angiogenesis, using in vitro analogues of
angiogenesis and an endothelial proliferation assay.

Method: Using an in vitro rat aortic ring assay we compared pseudotubule formation by medicinal honey (Activon), supermarket honey (Rowse) and a honey-based ointment (Mesitran), with that of artificial honey (70% w/w sugar glucose/fructose). Pseudotubules were analysed using TCS Cellworks AngioSys software. The Angiokit sytem was used to validate the results. Using the MTT [3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium. Bromide] assay, toxicity was also assessed on human umbilical vein endothelial cells (HUVEC) directly adherent to plastic.
Results: All honey preparations stimulated pseudotubule formation, maximal at around 0.2% honey. Medicinal honeys were more active than Rowse. The effect was not attributable to the sugar content. Among the honeys tested, the Manuka-based Activon preparation reduced residual viable biomass compared with a sugar control at >0.32% v/v concentration. Rowse had a similar effect only at 2.5%, the highest dose tested.

Conclusion: The influence of honey constituents on angiogenesis in a wound dressing context is likely to be positive, but would depend on the effective dilution of the honey and the penetration of the active constituents against an osmotic gradient. The extent to which this occurs has yet to be established.

Conflict of interest: This work was conceived, designed and executed by the authors. Medical honey preparations were supplied unconditionally but free of charge by the distributors.

Item Type: Article
ISSNs: 0969-0700 (print)
Related URLs:
Subjects: Q Science > QR Microbiology > QR355 Virology
R Medicine > RB Pathology
R Medicine > RM Therapeutics. Pharmacology
Divisions : University Structure - Pre August 2011 > School of Health Sciences
ePrint ID: 165675
Accepted Date and Publication Date:
14 October 2010Published
Date Deposited: 19 Oct 2010 08:31
Last Modified: 31 Mar 2016 13:29
URI: http://eprints.soton.ac.uk/id/eprint/165675

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