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A photoactivatable marker protein for pulse-chase imaging with superresolution

A photoactivatable marker protein for pulse-chase imaging with superresolution
A photoactivatable marker protein for pulse-chase imaging with superresolution
IrisFP is a photoactivatable fluorescent protein that combines irreversible photoconversion from a green- to a red-emitting form with reversible photoswitching between a fluorescent and a nonfluorescent state in both forms. Here we introduce a monomeric variant, mIrisFP, and demonstrate how its multiple photoactivation modes can be used for pulse-chase experiments combined with subdiffraction-resolution imaging in living cells by using dual-color photoactivation localization microscopy (PALM).
1548-7091
627-630
Fuchs, Jochen
a27d067e-7443-49a5-96c0-b6c2fb54b7fe
Böhme, Susan
c52de0a2-6970-476c-a1d6-c45142d0fc3c
Oswald, Franz
a5b02f2d-8439-411b-b5ad-999629cee58f
Hedde, Per Niklas
76e6eea2-ba66-4ed9-b85e-2b69c97b5c41
Krause, Maike
b98f1ad3-bac4-4dfd-9700-98206079355f
Wiedenmann, Jörg
ad445af2-680f-4927-90b3-589ac9d538f7
Nienhaus, G. Ulrich
64eb2ac6-4fa9-416c-a066-f096d79307cb
Fuchs, Jochen
a27d067e-7443-49a5-96c0-b6c2fb54b7fe
Böhme, Susan
c52de0a2-6970-476c-a1d6-c45142d0fc3c
Oswald, Franz
a5b02f2d-8439-411b-b5ad-999629cee58f
Hedde, Per Niklas
76e6eea2-ba66-4ed9-b85e-2b69c97b5c41
Krause, Maike
b98f1ad3-bac4-4dfd-9700-98206079355f
Wiedenmann, Jörg
ad445af2-680f-4927-90b3-589ac9d538f7
Nienhaus, G. Ulrich
64eb2ac6-4fa9-416c-a066-f096d79307cb

Fuchs, Jochen, Böhme, Susan, Oswald, Franz, Hedde, Per Niklas, Krause, Maike, Wiedenmann, Jörg and Nienhaus, G. Ulrich (2010) A photoactivatable marker protein for pulse-chase imaging with superresolution. Nature Methods, 7 (8), 627-630. (doi:10.1038/nmeth.1477).

Record type: Article

Abstract

IrisFP is a photoactivatable fluorescent protein that combines irreversible photoconversion from a green- to a red-emitting form with reversible photoswitching between a fluorescent and a nonfluorescent state in both forms. Here we introduce a monomeric variant, mIrisFP, and demonstrate how its multiple photoactivation modes can be used for pulse-chase experiments combined with subdiffraction-resolution imaging in living cells by using dual-color photoactivation localization microscopy (PALM).

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Published date: 2010
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Identifiers

Local EPrints ID: 168175
URI: http://eprints.soton.ac.uk/id/eprint/168175
DOI: doi:10.1038/nmeth.1477
ISSN: 1548-7091
PURE UUID: e69899a1-25fa-4f7e-88de-821707e2688c
ORCID for Jörg Wiedenmann: ORCID iD orcid.org/0000-0003-2128-2943

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Date deposited: 25 Nov 2010 09:30
Last modified: 14 Mar 2024 02:52

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Contributors

Author: Jochen Fuchs
Author: Susan Böhme
Author: Franz Oswald
Author: Per Niklas Hedde
Author: Maike Krause
Author: Jörg Wiedenmann ORCID iD
Author: G. Ulrich Nienhaus

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