Production of siderophore type chelates in Atlantic Ocean waters enriched with different carbon and nitrogen sources


Mawji, Edward, Gledhill, M., Milton, J.A., Zubkov, M.V., Thompson, Anu, Wolff, George A. and Achterberg, Eric P. (2011) Production of siderophore type chelates in Atlantic Ocean waters enriched with different carbon and nitrogen sources. Marine Chemistry, 124, (1-4), 90-99 . (doi:10.1016/j.marchem.2010.12.005).

Download

[img] PDF - Pre print
Download (601Kb)

Description/Abstract

Siderophore type chelates were detected in nutrient enriched, incubated seawater collected from different biogeographical regions of the Atlantic Ocean. Seawater was enriched with glucose and ammonium, glycine (as a source of carbon and nitrogen) or chitin and ammonium at different concentrations and incubated for up to 3 – 4 days in the dark. Siderophore type chelates were detected using high performance liquid chromatography coupled to inductively coupled plasma mass spectrometry (HPLC-ICP-MS) after complexation with Ga. Samples were subsequently analysed by HPLC - electrospray ionisation mass spectrometry (HPLC
ESI-MS) in order to confirm the identity of the known siderophores, and to obtain the pseudo molecular ions of unknown siderophore type chelates. A total of 22 different siderophore type chelates were resolved in the HPLC-ICP-MS chromatograms. Ten different siderophore type chelates were identified by HPLC-ESI-MS, 3 of which had not previously been identified in nutrient enriched seawater incubations. The concentration and diversity of siderophore typechelates was highest in seawater amended with glucose. The concentrations 1 and diversity of siderophore type chelates also varied with biogeographical area in the Atlantic Ocean, with the North Atlantic Sub-tropical Gyre yielding highest concentrations in incubations, and the South Atlantic Sub-tropical Gyre and Western Tropical Atlantic yielding the highest diversity.

Item Type: Article
ISSNs: 0304-4203
Keywords: Iron biogeochemistry; Siderophores; Seawater; Bacteria; Atlantic Ocean
Subjects: G Geography. Anthropology. Recreation > GC Oceanography
Q Science > QD Chemistry
Divisions: University Structure - Pre August 2011 > National Oceanography Centre (NERC)
University Structure - Pre August 2011 > School of Ocean & Earth Science (SOC/SOES)
National Oceanography Centre (NERC) > Ocean Biogeochemistry and Ecosystems
ePrint ID: 170213
Date Deposited: 04 Jan 2011 14:57
Last Modified: 27 Mar 2014 19:19
URI: http://eprints.soton.ac.uk/id/eprint/170213

Actions (login required)

View Item View Item

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics