Electroporation and lysis of marine microalga Karenia brevis for RNA extraction and amplification
Bahi, M.M., Tsaloglou, M-N., Mowlem, M. and Morgan, H. (2011) Electroporation and lysis of marine microalga Karenia brevis for RNA extraction and amplification. Journal of The Royal Society Interface, 8, (57), 601-608. (doi:10.1098/rsif.2010.0445).
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Description/Abstract
We describe here a simple device for dielectrophoretic concentration of marine microalga Karenia brevis non-motile cells, followed by electric field-mediated lysis for RNA extraction. The lysate was purified using magnetic beads and pure RNA extracted. RNA quality was assessed off-chip by nucleic acid sequence-based amplification and the optimum conditions for lysis were determined. This procedure will form part of an integrated microfluidic system that is being developed with sub-systems for performing cell concentration and lysis, RNA extraction/purification and real-time quantitative RNA detection. The integrated system and its components could be used for a large range of applications including in situ harmful algal bloom detection, transcriptomics and point-of-care diagnostics.
| Item Type: | Article |
|---|---|
| ISSNs: | 1742-5662 (electronic) |
| Divisions: | University Structure - Pre August 2011 > National Oceanography Centre (NERC) University Structure - Pre August 2011 > School of Electronics and Computer Science National Oceanography Centre (NERC) > Ocean Technology and Engineering |
| Item ID: | 171055 |
| Date Deposited: | 13 Jan 2011 09:21 |
| Last Modified: | 18 Jun 2012 08:49 |
| Contributors: | Bahi, M.M. (Author) Tsaloglou, M-N. (Author) Mowlem, M. (Author) Morgan, H. (Author) |
| Date: | 2011 |
| Status: | Published |
| URI: | http://eprints.soton.ac.uk/id/eprint/171055 |
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