Generic protease detection technology for monitoring periodontal disease

Zheng, Xinwei, Cook, Joseph P., Watkinson, Michael, Yang, Shoufeng, Douglas, Ian, Rawlinson, Andrew and Krause, Steffi (2011) Generic protease detection technology for monitoring periodontal disease. Faraday Discussions, 149, 37-47. (doi:10.1039/C005364C).


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Periodontal diseases are inflammatory conditions that affect the supporting tissues of teeth and can lead to destruction of the bone support and ultimately tooth loss if untreated. Progression of periodontitis is usually site specific but not uniform, and currently there are no accurate clinical methods for distinguishing sites where there is active disease progression from sites that are quiescent. Consequently, unnecessary and costly treatment of periodontal sites that are not progressing may occur. Three proteases have been identified as suitable markers for distinguishing sites with active disease progression and quiescent sites: human neutrophil elastase, cathepsin G and MMP8. Generic sensor materials for the detection of these three proteases have been developed based on thin dextran hydrogel films cross-linked with peptides. Degradation of the hydrogel films was monitored using impedance measurements. The target proteases were detected in the clinically relevant range within a time frame of 3 min. Good specificity for different proteases was achieved by choosing appropriate peptide cross-linkers.

Item Type: Article
Digital Object Identifier (DOI): doi:10.1039/C005364C
ISSNs: 1359-6640 (print)
1364-5498 (electronic)
Subjects: R Medicine > RB Pathology
R Medicine > RK Dentistry
T Technology > TA Engineering (General). Civil engineering (General)
Divisions : University Structure - Pre August 2011 > School of Engineering Sciences > Engineering Materials & Surface Engineering
ePrint ID: 172045
Accepted Date and Publication Date:
Date Deposited: 24 Jan 2011 11:20
Last Modified: 31 Mar 2016 13:32

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