Crystallization and preliminary X-ray diffraction analysis of the protease from Southampton norovirus complexed with a Michael acceptor inhibitor
Hussey, R.J., Coates, L., Gill, R.S., Wright, J. Neville, Sarwar, M., Coker, S., Erskine, P.T., Cooper, J.B., Wood, S.P., Clarke, I.N., Lambden, P.R., Broadbridge, R. and Shoolingin-Jordan, P.M. (2010) Crystallization and preliminary X-ray diffraction analysis of the protease from Southampton norovirus complexed with a Michael acceptor inhibitor. Acta Crystallographica. Section F, Structural Biology and Crystallization Communications, 66, (11), 1544-1548. (doi:10.1107/S1744309110039059). (PMID:21045318).
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Description/Abstract
Noroviruses are the predominant cause of human epidemic nonbacterial gastroenteritis. Viral replication requires a cysteine protease that cleaves a 200 kDa viral polyprotein into its constituent functional parts. Here, the crystallization of the recombinant protease from the Southampton norovirus is described. Whilst the native crystals were found to diffract only to medium resolution (2.9 Å), cocrystals of an inhibitor complex diffracted X-rays to 1.7 Å resolution. The polypeptide inhibitor (Ac-EFQLQ-propenyl ethyl ester) possesses an amino-acid sequence designed to match the substrate specificity of the enzyme, but was synthesized with a reactive Michael acceptor group at the C-terminal end.
| Item Type: | Article |
|---|---|
| ISSNs: | 1744-3091 (electronic) |
| Related URLs: | |
| Subjects: | Q Science > QR Microbiology > QR355 Virology R Medicine > RB Pathology |
| Divisions: | University Structure - Pre August 2011 > School of Medicine > Infection, Inflammation and Repair University Structure - Pre August 2011 > School of Biological Sciences |
| Item ID: | 173319 |
| Date Deposited: | 03 Feb 2011 11:14 |
| Last Modified: | 22 Aug 2012 10:44 |
| Contributors: | Hussey, R.J. (Author) Coates, L. (Author) Gill, R.S. (Author) Wright, J. Neville (Author) Sarwar, M. (Author) Coker, S. (Author) Erskine, P.T. (Author) Cooper, J.B. (Author) Wood, S.P. (Author) Clarke, I.N. (Author) Lambden, P.R. (Author) Broadbridge, R. (Author) Shoolingin-Jordan, P.M. (Author) |
| Date: | 1 November 2010 |
| Status: | Published |
| URI: | http://eprints.soton.ac.uk/id/eprint/173319 |
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