Conditional depletion of airway progenitor cells induces peribronchiolar fibrosis

Perl, Anne-Karina T., Riethmacher, Dieter and Whitsett, Jeffrey A. (2011) Conditional depletion of airway progenitor cells induces peribronchiolar fibrosis. American Journal of Respiratory and Critical Care Medicine, 183, (4), 511-521. (doi:10.1164/rccm.201005-0744OC). (PMID:20870756).


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RATIONALE: The respiratory epithelium has a remarkable capacity to respond to acute injury. In contrast, repeated epithelial injury is often associated with abnormal repair, inflammation and fibrosis. There is increasing evidence that non-ciliated epithelial cells play important roles in the repair of the bronchiolar epithelium following acute injury. Cellular processes underlying the repair and remodeling of the lung following chronic epithelial injury are poorly understood. OBJECTIVES: To identify cell processes mediating epithelial regeneration and remodeling after acute and chronic Clara cell depletion. METHODS: A transgenic mouse model was generated to conditionally express diphtheria toxin A to ablate Clara cells in the adult lung. Epithelial regeneration and peribronchiolar fibrosis were assessed after acute and chronic Clara cell depletion. MEASUREMENTS AND MAIN RESULTS: Acute Clara cell ablation caused squamous metaplasia of ciliated cells and induced proliferation of residual progenitor cells. Ciliated cells in the bronchioles and pro-SPC expressing cells in the bronchiolar alveolar duct junctions did not proliferate. Epithelial cell proliferation occurred at multiple sites along the airways and was not selectively associated with regions around neuroepithelial bodies. Chronic Clara cell depletion resulted in ineffective repair and caused peribronchiolar fibrosis. CONCLUSIONS: Co-localization of proliferation and cell type specific markers demonstrate that Clara cells are critical airway progenitor cells. Continuous depletion of Clara cells resulted in persistent squamous metaplasia, lack of normal re-epithelialization and peribronchiolar fibrosis. Induction of proliferation in subepithelial fibroblasts, supports the concept that chronic epithelial depletion caused peribronchiolar fibrosis.

Item Type: Article
Digital Object Identifier (DOI): doi:10.1164/rccm.201005-0744OC
ISSNs: 1073-449X (print)
1535-4970 (electronic)
Keywords: chronic obstructive, squamous metaplasia, diphtheria toxin, progenitor cells
Subjects: Q Science > QP Physiology
R Medicine > RB Pathology
Divisions : University Structure - Pre August 2011 > School of Medicine > Human Genetics
ePrint ID: 174871
Accepted Date and Publication Date:
15 February 2011Published
24 September 2010Made publicly available
Date Deposited: 17 Feb 2011 15:12
Last Modified: 31 Mar 2016 13:33

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