Surface-bound myeloperoxidase is a ligand for recognition of late apoptotic neutrophils by human lung surfactant proteins A and D
Jakel, Anne, Clark, Howard, Reid, Kenneth and Sim, Robert B. (2010) Surface-bound myeloperoxidase is a ligand for recognition of late apoptotic neutrophils by human lung surfactant proteins A and D. Protein & Cell, 1, (6), 563-572. (doi:10.1007/s13238-010-0076-0). (PMID:21204009).
Download
Full text not available from this repository.
Description/Abstract
Surfactant proteins A (SP-A) and D (SP-D), both members of the collectin family, play a well established role in apoptotic cell recognition and clearance. Recent in vitro data show that SP-A and SP-D interact with apoptotic neutrophils in a distinct manner. SP-A and SP-D bind in a Ca2+-dependent manner to viable and early apoptotic neutrophils whereas the much greater interaction with late apoptotic neutrophils is Ca2+-independent. Cell surface molecules on the apoptotic target cells responsible for these interactions had not been identified and this study was done to find candidate target molecules. Myeloperoxidase (MPO), a specific intracellular defense molecule of neutrophils that becomes exposed on the outside of the cell upon apoptosis, was identified by affinity purification, mass-spectrometry and western blotting as a novel binding molecule for SP-A and SP-D. To confirm its role in recognition, it was shown that purified immobilised MPO binds SP-A and SP-D, and that MPO is surface-exposed on late apoptotic neutrophils. SP-A and SP-D inhibit binding of an anti-MPO monoclonal Ab to late apoptotic cells. Fluorescence microscopy confirmed that anti-MPO mAb and SP-A/SP-D colocalise on late apoptotic neutrophils. Desmoplakin was identified as a further potential ligand for SP-A, and neutrophil defensin as a target for both proteins.
| Item Type: | Article |
|---|---|
| ISSNs: | 1674-800X (print) 1674-8018 (electronic) |
| Keywords: | sp-a, sp-d, myeloperoxidase, neutrophils, flow cytometry, mass spectrometry |
| Subjects: | Q Science > QH Natural history > QH301 Biology R Medicine > RC Internal medicine |
| Divisions: | University Structure - Pre August 2011 > School of Medicine |
| Item ID: | 176007 |
| Date Deposited: | 02 Mar 2011 11:20 |
| Last Modified: | 25 Apr 2013 23:45 |
| Contributors: | Jakel, Anne (Author) Clark, Howard (Author) Reid, Kenneth (Author) Sim, Robert B. (Author) |
| Date: | June 2010 |
| Status: | Published |
| URI: | http://eprints.soton.ac.uk/id/eprint/176007 |
Actions (login required)
 |
View Item |
