Controlled differentiation of human bone marrow stromal cells using magnetic nanoparticle technology
Controlled differentiation of human bone marrow stromal cells using magnetic nanoparticle technology
Targeting and differentiating stem cells at sites of injury and repair is an exciting and promising area for disease treatment and reparative medicine. We have investigated remote magnetic field activation of magnetic nanoparticle-tagged mechanosensitive receptors on the cell membrane of human bone marrow stromal cells (HBMSCs) for use in osteoprogenitor cell delivery systems and activation of differentiation in vitro and in vivo toward an osteochondral lineage. HBMSC-labeled with magnetic beads coated with antibodies or peptides to the transmembrane ion channel stretch activated potassium channel (TREK-1) or arginine–glycine–aspartic acid were cultured in monolayer or encapsulated into polysaccharide alginate/chitosan microcapsules. Upregulation in gene expression was measured in magnetic particle-labeled HBMSCs in response to TREK-1 activation over a short period (7 days) with an increase in mRNA levels of Sox9, core binding factor alpha1 (Cbfa1), and osteopontin. Magnetic particle-labeled HBMSCs encapsulated into alginate chitosan capsules were exposed to magnetic forces both in vitro and in vivo intermittently for 21 days. After 21 days the encapsulated, magnetic particle-labeled HBMSCs in vivo were viable as evidenced by extensive cell tracker green fluorescence. The mechanical stimulation of HBMSCs labeled with TREK-1 magnetic nanoparticle receptors enhanced expression of type-1 collagen in vitro with increases in proteoglycan matrix, core binding factor alpha1 (Cbfa1) and collagen synthesis, and extracellular matrix production and elevated the expression of type-1 and type-2 collagen in vivo. Additionally, the magnetically remote stimulation of HBMSCs labeled with magnetic nanoparticle arginine–glycine–aspartic acid considerably enhanced proteoglycan and collagen synthesis and extracellular matrix production and elevated the expression of type-1 and type-2 collagen in vivo and in vitro. Osteogenic mechanosensitive receptor manipulation by magnetic nanotechnology can induce the differentiation of osteoprogenitor cell populations toward an osteogenic lineage. These cell manipulation strategies offer tremendous therapeutic opportunities in soft and hard tissue repair.
3241-3250
Kanczler, Janos M.
eb8db9ff-a038-475f-9030-48eef2b0559c
Sura, Harpul S.
d8fe986b-793e-43c9-9294-2560d0ddd4bb
Magnay, Julia
d415d197-f537-45e5-903f-c3a26c5f4ef8
Green, David
ec0d912d-251d-47ef-82be-f2abd97e2183
Oreffo, Richard O. C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Dobson, Jon P.
fd24442a-0be3-46ef-8cf2-e25ba7f62525
El Haj, Alicia J.
40529e99-8b75-4d0e-b7fe-288c4177e3c3
October 2010
Kanczler, Janos M.
eb8db9ff-a038-475f-9030-48eef2b0559c
Sura, Harpul S.
d8fe986b-793e-43c9-9294-2560d0ddd4bb
Magnay, Julia
d415d197-f537-45e5-903f-c3a26c5f4ef8
Green, David
ec0d912d-251d-47ef-82be-f2abd97e2183
Oreffo, Richard O. C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Dobson, Jon P.
fd24442a-0be3-46ef-8cf2-e25ba7f62525
El Haj, Alicia J.
40529e99-8b75-4d0e-b7fe-288c4177e3c3
Kanczler, Janos M., Sura, Harpul S., Magnay, Julia, Green, David, Oreffo, Richard O. C., Dobson, Jon P. and El Haj, Alicia J.
(2010)
Controlled differentiation of human bone marrow stromal cells using magnetic nanoparticle technology.
Tissue Engineering Part A, 16 (10), .
(doi:10.1089/ten.tea.2009.0638).
(PMID:20504072)
Abstract
Targeting and differentiating stem cells at sites of injury and repair is an exciting and promising area for disease treatment and reparative medicine. We have investigated remote magnetic field activation of magnetic nanoparticle-tagged mechanosensitive receptors on the cell membrane of human bone marrow stromal cells (HBMSCs) for use in osteoprogenitor cell delivery systems and activation of differentiation in vitro and in vivo toward an osteochondral lineage. HBMSC-labeled with magnetic beads coated with antibodies or peptides to the transmembrane ion channel stretch activated potassium channel (TREK-1) or arginine–glycine–aspartic acid were cultured in monolayer or encapsulated into polysaccharide alginate/chitosan microcapsules. Upregulation in gene expression was measured in magnetic particle-labeled HBMSCs in response to TREK-1 activation over a short period (7 days) with an increase in mRNA levels of Sox9, core binding factor alpha1 (Cbfa1), and osteopontin. Magnetic particle-labeled HBMSCs encapsulated into alginate chitosan capsules were exposed to magnetic forces both in vitro and in vivo intermittently for 21 days. After 21 days the encapsulated, magnetic particle-labeled HBMSCs in vivo were viable as evidenced by extensive cell tracker green fluorescence. The mechanical stimulation of HBMSCs labeled with TREK-1 magnetic nanoparticle receptors enhanced expression of type-1 collagen in vitro with increases in proteoglycan matrix, core binding factor alpha1 (Cbfa1) and collagen synthesis, and extracellular matrix production and elevated the expression of type-1 and type-2 collagen in vivo. Additionally, the magnetically remote stimulation of HBMSCs labeled with magnetic nanoparticle arginine–glycine–aspartic acid considerably enhanced proteoglycan and collagen synthesis and extracellular matrix production and elevated the expression of type-1 and type-2 collagen in vivo and in vitro. Osteogenic mechanosensitive receptor manipulation by magnetic nanotechnology can induce the differentiation of osteoprogenitor cell populations toward an osteogenic lineage. These cell manipulation strategies offer tremendous therapeutic opportunities in soft and hard tissue repair.
This record has no associated files available for download.
More information
Published date: October 2010
Identifiers
Local EPrints ID: 176459
URI: http://eprints.soton.ac.uk/id/eprint/176459
ISSN: 1937-3341
PURE UUID: 63928395-ebb6-440e-9ee8-f09b4b0df613
Catalogue record
Date deposited: 09 Mar 2011 15:28
Last modified: 14 Mar 2024 02:50
Export record
Altmetrics
Contributors
Author:
Janos M. Kanczler
Author:
Harpul S. Sura
Author:
Julia Magnay
Author:
David Green
Author:
Jon P. Dobson
Author:
Alicia J. El Haj
Download statistics
Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.
View more statistics