Receptor-mediated interactions between colloidal gold nanoparticles and human umbilical vein endothelial cells
Receptor-mediated interactions between colloidal gold nanoparticles and human umbilical vein endothelial cells
A new strategy to manipulate cell operations is demonstrated, based on membrane-receptor-specific interactions between colloidal peptide-capped gold nanoparticles and human umbilical vein endothelial cells. It is shown that colloidal gold nanoparticles of similar charge and size but capped with different peptide sequences can deliberately trigger specific cell functions related to the important biological process of blood vessel growth known as angiogenesis. Specific binding of the peptide-capped particles to two endothelial-expressed receptors (VEGFR-1, NRP-1), which control angiogenesis, is achieved. The cellular fate of the functional nanoparticles is imaged and the influence of the different peptide-coated nanoparticles on the gene expression profile of hypoxia-related and angiogenic genes is monitored. The findings open up new avenues towards the deliberate biological control of cellular functions using strategically designed nanoparticles
cells, gene expression, gold, nanoparticles, peptides
388-94
Bartczak, Dorota
4d706d11-ee17-4e30-9ba8-1d4b3d878f71
Sanchez-Elsner, Tilman
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Louafi, Fethi
75af1544-3c81-4a94-9bd3-50c2c74ad5c4
Millar, Timothy M.
ec88510c-ad88-49f6-8b2d-4277c84c1958
Kanaras, Antonios G.
667ecfdc-7647-4bd8-be03-a47bf32504c7
7 February 2011
Bartczak, Dorota
4d706d11-ee17-4e30-9ba8-1d4b3d878f71
Sanchez-Elsner, Tilman
b8799f8d-e2b4-4b37-b77c-f2f0e8e2070d
Louafi, Fethi
75af1544-3c81-4a94-9bd3-50c2c74ad5c4
Millar, Timothy M.
ec88510c-ad88-49f6-8b2d-4277c84c1958
Kanaras, Antonios G.
667ecfdc-7647-4bd8-be03-a47bf32504c7
Bartczak, Dorota, Sanchez-Elsner, Tilman, Louafi, Fethi, Millar, Timothy M. and Kanaras, Antonios G.
(2011)
Receptor-mediated interactions between colloidal gold nanoparticles and human umbilical vein endothelial cells.
Small, 7 (3), .
(doi:10.1002/smll.201001816).
Abstract
A new strategy to manipulate cell operations is demonstrated, based on membrane-receptor-specific interactions between colloidal peptide-capped gold nanoparticles and human umbilical vein endothelial cells. It is shown that colloidal gold nanoparticles of similar charge and size but capped with different peptide sequences can deliberately trigger specific cell functions related to the important biological process of blood vessel growth known as angiogenesis. Specific binding of the peptide-capped particles to two endothelial-expressed receptors (VEGFR-1, NRP-1), which control angiogenesis, is achieved. The cellular fate of the functional nanoparticles is imaged and the influence of the different peptide-coated nanoparticles on the gene expression profile of hypoxia-related and angiogenic genes is monitored. The findings open up new avenues towards the deliberate biological control of cellular functions using strategically designed nanoparticles
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Published date: 7 February 2011
Keywords:
cells, gene expression, gold, nanoparticles, peptides
Organisations:
Quantum, Light & Matter Group
Identifiers
Local EPrints ID: 178467
URI: http://eprints.soton.ac.uk/id/eprint/178467
ISSN: 1613-6810
PURE UUID: 879f99b5-90df-4850-ac91-9c77186b1ef1
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Date deposited: 25 Mar 2011 10:26
Last modified: 14 Mar 2024 02:53
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Contributors
Author:
Dorota Bartczak
Author:
Fethi Louafi
Author:
Timothy M. Millar
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