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Phosphorylation of RIG-I by casein kinase II inhibits its antiviral response

Phosphorylation of RIG-I by casein kinase II inhibits its antiviral response
Phosphorylation of RIG-I by casein kinase II inhibits its antiviral response
RIG-I is an intracellular RNA virus sensor that mediates a signaling pathway that triggers the alpha/beta interferon (IFN-?/?) immune defenses. However, the mechanism for regulation of RIG-I activity remains largely unknown. Here we show that RIG-I activity is regulated by phosphorylation and dephosphorylation in its repressor domain (RD). Threonine at amino acid (aa) 770 and serine at aa 854 to 855 of RIG-I are phosphorylated by casein kinase II (CK2) in the resting state of the cell and dephosphorylated when cells are infected by RNA virus. Mutation at aa position 770 or 854 to 855 of RIG-I renders it constitutively active. Pharmacological inhibition of CK2 enhances virus-induced expression of IFN-? and suppresses virus proliferation, while inhibition of phosphatase reduces virus-induced expression of IFN-?. Overexpression of CK2 suppresses RIG-I-mediated signaling, while silencing of CK2 results in the increased suppression of virus proliferation. Our results reveal a novel mechanism of the regulation of RIG-I activity during RNA virus infection.
0022-538X
1036-1047
Sun, Zhiguo
16cd3391-7784-4f84-94bc-c03ed06eb07f
Ren, Hongwei
53542f2c-e294-4e26-a581-344e646c72e6
Liu, Yan
3d2550f3-df3b-46fd-a49e-511f7abe6424
Teeling, Jessica L
fcde1c8e-e5f8-4747-9f3a-6bdb5cd87d0a
Gu, Jun
fd0220da-8019-4327-92ad-fcadeb9896b4
Sun, Zhiguo
16cd3391-7784-4f84-94bc-c03ed06eb07f
Ren, Hongwei
53542f2c-e294-4e26-a581-344e646c72e6
Liu, Yan
3d2550f3-df3b-46fd-a49e-511f7abe6424
Teeling, Jessica L
fcde1c8e-e5f8-4747-9f3a-6bdb5cd87d0a
Gu, Jun
fd0220da-8019-4327-92ad-fcadeb9896b4

Sun, Zhiguo, Ren, Hongwei, Liu, Yan, Teeling, Jessica L and Gu, Jun (2011) Phosphorylation of RIG-I by casein kinase II inhibits its antiviral response. Journal of Virology, 85 (2), 1036-1047. (doi:10.1128/JVI.01734-10). (PMID:21068236)

Record type: Article

Abstract

RIG-I is an intracellular RNA virus sensor that mediates a signaling pathway that triggers the alpha/beta interferon (IFN-?/?) immune defenses. However, the mechanism for regulation of RIG-I activity remains largely unknown. Here we show that RIG-I activity is regulated by phosphorylation and dephosphorylation in its repressor domain (RD). Threonine at amino acid (aa) 770 and serine at aa 854 to 855 of RIG-I are phosphorylated by casein kinase II (CK2) in the resting state of the cell and dephosphorylated when cells are infected by RNA virus. Mutation at aa position 770 or 854 to 855 of RIG-I renders it constitutively active. Pharmacological inhibition of CK2 enhances virus-induced expression of IFN-? and suppresses virus proliferation, while inhibition of phosphatase reduces virus-induced expression of IFN-?. Overexpression of CK2 suppresses RIG-I-mediated signaling, while silencing of CK2 results in the increased suppression of virus proliferation. Our results reveal a novel mechanism of the regulation of RIG-I activity during RNA virus infection.

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Published date: January 2011

Identifiers

Local EPrints ID: 180219
URI: http://eprints.soton.ac.uk/id/eprint/180219
ISSN: 0022-538X
PURE UUID: 0dbe7018-fcc3-4ceb-9cb2-f7ce3cc23fb0
ORCID for Jessica L Teeling: ORCID iD orcid.org/0000-0003-4004-7391

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Date deposited: 06 Apr 2011 11:18
Last modified: 15 Mar 2024 03:21

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Contributors

Author: Zhiguo Sun
Author: Hongwei Ren
Author: Yan Liu
Author: Jun Gu

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