Beta-hydroxy-beta-methylbutyrate modifies human peripheral blood mononuclear cell proliferation and cytokine production in vitro
Beta-hydroxy-beta-methylbutyrate modifies human peripheral blood mononuclear cell proliferation and cytokine production in vitro
Objective: the main objective was to investigate the potential immunomodulatory effects of ?-hydroxy-?-methylbutyrate (HMB) in human cells.
Methods: peripheral blood mononuclear cells were isolated from the blood of eight volunteers and assayed for proliferation, cell cycle progression, surface expression of CD25, intracellular expression of pERK1/2, and cytokine production after in vitro exposure to a range of HMB concentrations (0.1 to 10 mM).
Results: above 1 mM, HMB decreased the extent of proliferation normally observed after stimulation by concanavalin A. The decrease was evident at 10 mM HMB, when the proliferation index was 50% reduced when compared with the absence of HMB. Cell cycle analysis demonstrated an increase in the proportion of cells at the G0-G1 phase at 10 mM HMB. CD25 and pERK1/2 expression were not related to the observed effect on proliferation. HMB affected the concentrations of all five cytokines measured following stimulation. Tumor necrosis factor-? concentration in the culture medium was reduced by not, vert at all HMB concentrations. Th1/Th2 cytokine production was modified toward a Th2 profile when HMB was at 1 or 10 mM. Thus, HMB at 10 mM impairs lymphocyte proliferation and progression through the cell cycle. The lowest concentration used here (0.1 mM) exerted some actions on cytokine production, including decreasing TNF-? production, but not on proliferation and cell cycle progression.
Conclusion: HMB may be a useful agent to consider for modulation of immune function in specific situations
hmb, cytokines, lymphocyte, proliferation, immunomodulation, cell cycle
92-99
Nunes, Everson A.
62a7f5db-40d7-4814-a1d0-2c1eb71a9c29
Lomax, Amy R.
8c33168f-c3e7-4c3e-a579-dad99a866d07
Noakes, Paul S.
0ed50cd9-de73-4851-8039-ee72860d8ae5
Miles, Elizabeth A.
20332899-ecdb-4214-95bc-922dde36d416
Fernandes, Luiz C.
380a7161-4e23-40d3-afde-7cc36ec65060
Calder, Phillip C.
1797e54f-378e-4dcb-80a4-3e30018f07a6
January 2011
Nunes, Everson A.
62a7f5db-40d7-4814-a1d0-2c1eb71a9c29
Lomax, Amy R.
8c33168f-c3e7-4c3e-a579-dad99a866d07
Noakes, Paul S.
0ed50cd9-de73-4851-8039-ee72860d8ae5
Miles, Elizabeth A.
20332899-ecdb-4214-95bc-922dde36d416
Fernandes, Luiz C.
380a7161-4e23-40d3-afde-7cc36ec65060
Calder, Phillip C.
1797e54f-378e-4dcb-80a4-3e30018f07a6
Nunes, Everson A., Lomax, Amy R., Noakes, Paul S., Miles, Elizabeth A., Fernandes, Luiz C. and Calder, Phillip C.
(2011)
Beta-hydroxy-beta-methylbutyrate modifies human peripheral blood mononuclear cell proliferation and cytokine production in vitro.
Nutrition, 27 (1), .
(doi:10.1016/j.nut.2009.12.008).
(PMID:20541366)
Abstract
Objective: the main objective was to investigate the potential immunomodulatory effects of ?-hydroxy-?-methylbutyrate (HMB) in human cells.
Methods: peripheral blood mononuclear cells were isolated from the blood of eight volunteers and assayed for proliferation, cell cycle progression, surface expression of CD25, intracellular expression of pERK1/2, and cytokine production after in vitro exposure to a range of HMB concentrations (0.1 to 10 mM).
Results: above 1 mM, HMB decreased the extent of proliferation normally observed after stimulation by concanavalin A. The decrease was evident at 10 mM HMB, when the proliferation index was 50% reduced when compared with the absence of HMB. Cell cycle analysis demonstrated an increase in the proportion of cells at the G0-G1 phase at 10 mM HMB. CD25 and pERK1/2 expression were not related to the observed effect on proliferation. HMB affected the concentrations of all five cytokines measured following stimulation. Tumor necrosis factor-? concentration in the culture medium was reduced by not, vert at all HMB concentrations. Th1/Th2 cytokine production was modified toward a Th2 profile when HMB was at 1 or 10 mM. Thus, HMB at 10 mM impairs lymphocyte proliferation and progression through the cell cycle. The lowest concentration used here (0.1 mM) exerted some actions on cytokine production, including decreasing TNF-? production, but not on proliferation and cell cycle progression.
Conclusion: HMB may be a useful agent to consider for modulation of immune function in specific situations
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Published date: January 2011
Keywords:
hmb, cytokines, lymphocyte, proliferation, immunomodulation, cell cycle
Organisations:
Dev Origins of Health & Disease
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Local EPrints ID: 180649
URI: http://eprints.soton.ac.uk/id/eprint/180649
ISSN: 0899-9007
PURE UUID: b14976f0-1edf-41fe-9ceb-128446357f17
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Date deposited: 12 Apr 2011 14:18
Last modified: 15 Mar 2024 03:27
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Author:
Everson A. Nunes
Author:
Amy R. Lomax
Author:
Paul S. Noakes
Author:
Luiz C. Fernandes
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