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Beta-hydroxy-beta-methylbutyrate modifies human peripheral blood mononuclear cell proliferation and cytokine production in vitro

Beta-hydroxy-beta-methylbutyrate modifies human peripheral blood mononuclear cell proliferation and cytokine production in vitro
Beta-hydroxy-beta-methylbutyrate modifies human peripheral blood mononuclear cell proliferation and cytokine production in vitro
Objective: the main objective was to investigate the potential immunomodulatory effects of ?-hydroxy-?-methylbutyrate (HMB) in human cells.

Methods: peripheral blood mononuclear cells were isolated from the blood of eight volunteers and assayed for proliferation, cell cycle progression, surface expression of CD25, intracellular expression of pERK1/2, and cytokine production after in vitro exposure to a range of HMB concentrations (0.1 to 10 mM).

Results: above 1 mM, HMB decreased the extent of proliferation normally observed after stimulation by concanavalin A. The decrease was evident at 10 mM HMB, when the proliferation index was 50% reduced when compared with the absence of HMB. Cell cycle analysis demonstrated an increase in the proportion of cells at the G0-G1 phase at 10 mM HMB. CD25 and pERK1/2 expression were not related to the observed effect on proliferation. HMB affected the concentrations of all five cytokines measured following stimulation. Tumor necrosis factor-? concentration in the culture medium was reduced by not, vert at all HMB concentrations. Th1/Th2 cytokine production was modified toward a Th2 profile when HMB was at 1 or 10 mM. Thus, HMB at 10 mM impairs lymphocyte proliferation and progression through the cell cycle. The lowest concentration used here (0.1 mM) exerted some actions on cytokine production, including decreasing TNF-? production, but not on proliferation and cell cycle progression.

Conclusion: HMB may be a useful agent to consider for modulation of immune function in specific situations
hmb, cytokines, lymphocyte, proliferation, immunomodulation, cell cycle
0899-9007
92-99
Nunes, Everson A.
62a7f5db-40d7-4814-a1d0-2c1eb71a9c29
Lomax, Amy R.
8c33168f-c3e7-4c3e-a579-dad99a866d07
Noakes, Paul S.
0ed50cd9-de73-4851-8039-ee72860d8ae5
Miles, Elizabeth A.
20332899-ecdb-4214-95bc-922dde36d416
Fernandes, Luiz C.
380a7161-4e23-40d3-afde-7cc36ec65060
Calder, Phillip C.
1797e54f-378e-4dcb-80a4-3e30018f07a6
Nunes, Everson A.
62a7f5db-40d7-4814-a1d0-2c1eb71a9c29
Lomax, Amy R.
8c33168f-c3e7-4c3e-a579-dad99a866d07
Noakes, Paul S.
0ed50cd9-de73-4851-8039-ee72860d8ae5
Miles, Elizabeth A.
20332899-ecdb-4214-95bc-922dde36d416
Fernandes, Luiz C.
380a7161-4e23-40d3-afde-7cc36ec65060
Calder, Phillip C.
1797e54f-378e-4dcb-80a4-3e30018f07a6

Nunes, Everson A., Lomax, Amy R., Noakes, Paul S., Miles, Elizabeth A., Fernandes, Luiz C. and Calder, Phillip C. (2011) Beta-hydroxy-beta-methylbutyrate modifies human peripheral blood mononuclear cell proliferation and cytokine production in vitro. Nutrition, 27 (1), 92-99. (doi:10.1016/j.nut.2009.12.008). (PMID:20541366)

Record type: Article

Abstract

Objective: the main objective was to investigate the potential immunomodulatory effects of ?-hydroxy-?-methylbutyrate (HMB) in human cells.

Methods: peripheral blood mononuclear cells were isolated from the blood of eight volunteers and assayed for proliferation, cell cycle progression, surface expression of CD25, intracellular expression of pERK1/2, and cytokine production after in vitro exposure to a range of HMB concentrations (0.1 to 10 mM).

Results: above 1 mM, HMB decreased the extent of proliferation normally observed after stimulation by concanavalin A. The decrease was evident at 10 mM HMB, when the proliferation index was 50% reduced when compared with the absence of HMB. Cell cycle analysis demonstrated an increase in the proportion of cells at the G0-G1 phase at 10 mM HMB. CD25 and pERK1/2 expression were not related to the observed effect on proliferation. HMB affected the concentrations of all five cytokines measured following stimulation. Tumor necrosis factor-? concentration in the culture medium was reduced by not, vert at all HMB concentrations. Th1/Th2 cytokine production was modified toward a Th2 profile when HMB was at 1 or 10 mM. Thus, HMB at 10 mM impairs lymphocyte proliferation and progression through the cell cycle. The lowest concentration used here (0.1 mM) exerted some actions on cytokine production, including decreasing TNF-? production, but not on proliferation and cell cycle progression.

Conclusion: HMB may be a useful agent to consider for modulation of immune function in specific situations

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More information

Published date: January 2011
Keywords: hmb, cytokines, lymphocyte, proliferation, immunomodulation, cell cycle
Organisations: Dev Origins of Health & Disease

Identifiers

Local EPrints ID: 180649
URI: http://eprints.soton.ac.uk/id/eprint/180649
ISSN: 0899-9007
PURE UUID: b14976f0-1edf-41fe-9ceb-128446357f17
ORCID for Paul S. Noakes: ORCID iD orcid.org/0000-0002-2678-1971
ORCID for Elizabeth A. Miles: ORCID iD orcid.org/0000-0002-8643-0655
ORCID for Phillip C. Calder: ORCID iD orcid.org/0000-0002-6038-710X

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Date deposited: 12 Apr 2011 14:18
Last modified: 15 Mar 2024 03:27

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Contributors

Author: Everson A. Nunes
Author: Amy R. Lomax
Author: Paul S. Noakes ORCID iD
Author: Luiz C. Fernandes

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