Trapping single human osteoblast-like cells from a heterogeneous population using a dielectrophoretic microfluidic device
Thomas, Rupert S.W., Mitchell, Peter D., Oreffo, Richard O. and Morgan, Hywel (2010) Trapping single human osteoblast-like cells from a heterogeneous population using a dielectrophoretic microfluidic device. [in special issue: Dielectrophoresis] Biomicrofluidics, 4, (2), 022806. (doi:10.1063/1.3406951). (PMID:20697594). (Submitted).
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We describe a system for the isolation, concentration, separation, and recovery of human osteoblast-like cells from a heterogeneous population using dielectrophoretic ring traps. Cells flowing in a microfluidic channel are immobilized inside an electric field cage using negative dielectrophoresis. A planar ring electrode creates a closed trap while repelling surrounding cells. Target cells are identified by fluorescent labeling, and are trapped as they pass across a ring electrode by an automated system. We demonstrate recovery of small populations of human osteoblast-like cells with a purity of 100%, which in turn demonstrates the potential of such a device for cell selection from a heterogeneous population.
|Digital Object Identifier (DOI):||doi:10.1063/1.3406951|
|Subjects:||Q Science > QR Microbiology
R Medicine > RB Pathology
T Technology > TK Electrical engineering. Electronics Nuclear engineering
|Divisions:||University Structure - Pre August 2011 > School of Medicine > Developmental Origins of Health and Disease
|Date Deposited:||18 Apr 2011 14:35|
|Last Modified:||14 Apr 2014 10:57|
Combining stem cell science and tissue engineering to study the development and repair of human skeletal tissue
Funded by: BBSRC (BB/G010579/1)
Led by: Richard Oreffo
1 October 2009 to 30 September 2014
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