Trapping single human osteoblast-like cells from a heterogeneous population using a dielectrophoretic microfluidic device
Thomas, Rupert S.W., Mitchell, Peter D., Oreffo, Richard O. and Morgan, Hywel (2010) Trapping single human osteoblast-like cells from a heterogeneous population using a dielectrophoretic microfluidic device. [in special issue: Dielectrophoresis] Biomicrofluidics, 4, (2), 022806. (doi:10.1063/1.3406951). (PMID:20697594). (Submitted).
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Description/Abstract
We describe a system for the isolation, concentration, separation, and recovery of human osteoblast-like cells from a heterogeneous population using dielectrophoretic ring traps. Cells flowing in a microfluidic channel are immobilized inside an electric field cage using negative dielectrophoresis. A planar ring electrode creates a closed trap while repelling surrounding cells. Target cells are identified by fluorescent labeling, and are trapped as they pass across a ring electrode by an automated system. We demonstrate recovery of small populations of human osteoblast-like cells with a purity of 100%, which in turn demonstrates the potential of such a device for cell selection from a heterogeneous population.
| Item Type: | Article |
|---|---|
| ISSNs: | 1932-1058 (print) 1932-1058 (electronic) |
| Subjects: | Q Science > QR Microbiology R Medicine > RB Pathology T Technology > TK Electrical engineering. Electronics Nuclear engineering |
| Divisions: | University Structure - Pre August 2011 > School of Medicine > Developmental Origins of Health and Disease |
| Item ID: | 181661 |
| Date Deposited: | 18 Apr 2011 14:35 |
| Last Modified: | 01 Jun 2011 01:19 |
| Contributors: | Thomas, Rupert S.W. (Author) Mitchell, Peter D. (Author) Oreffo, Richard O. (Author) Morgan, Hywel (Author) |
| Date: | June 2010 |
| Status: | Submitted |
| URI: | http://eprints.soton.ac.uk/id/eprint/181661 |
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