Whole proteome analysis of osteoprogenitor differentiation induced by disordered nanotopography and mediated by ERK signalling
Whole proteome analysis of osteoprogenitor differentiation induced by disordered nanotopography and mediated by ERK signalling
Topographic features can modulate cell behaviours such as proliferation, migration, differentiation and apoptosis. Biochemical mechanotransduction implies the conversion of mechanical forces (e.g. changes in cell spreading and morphology from changing surface topography) into biochemical signal via biomolecules. Still, little is known concerning which pathways may be directly involved in cell response to changes in the material surface. A number of pathways have been implicated using focused studies of ‘selected’ biomolecules rather than a global analysis of signal pathways. This study used a controlled disorder nanopit topography (NSQ50, fabricated by electron beam lithography) to direct osteoblast differentiation of progenitor cells. This topography is unique as it represents a middle route (from absolute order or random roughness) that allows osteoconversion with similar efficiency as dexamethasone and ascorbate treatment. Two direct-comparison proteomics techniques, firstly gel-based and then chromatography-based, were used to analyse progenitor proteome changes in response to the nanotopography. Many of the changed proteins form part of the Extracellular Signal-regulated Kinase (ERK1/2) pathway.
tissue engineering, disordered topography, extracellular-activated protein kinase, osteoprogenitor cells, proteomics
4723-4732
Kantawong, Fahsai
d209298e-ac00-49f0-a9b9-39d1e82d5925
Burgess, Karl E.V.
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Jayawardena, Kamburapola
0481e755-3e8d-42e0-947e-895de802c3f8
Hart, Andrew
6011e639-937a-4d81-aa83-ba1ad25ca916
Burchmore, Richard J.
9db5ecb2-23e8-4a50-ad42-c41d3c414306
Gadegaard, Nikolaj
bf72c3d1-e6a7-48b4-a968-c496b655bd98
Oreffo, Richard O.C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Dalby, Matthew J.
25dcae6a-8289-4169-abb7-c45fff0bafdc
September 2009
Kantawong, Fahsai
d209298e-ac00-49f0-a9b9-39d1e82d5925
Burgess, Karl E.V.
7eef15f0-15b2-4898-ac8a-bb5f49f8ff48
Jayawardena, Kamburapola
0481e755-3e8d-42e0-947e-895de802c3f8
Hart, Andrew
6011e639-937a-4d81-aa83-ba1ad25ca916
Burchmore, Richard J.
9db5ecb2-23e8-4a50-ad42-c41d3c414306
Gadegaard, Nikolaj
bf72c3d1-e6a7-48b4-a968-c496b655bd98
Oreffo, Richard O.C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Dalby, Matthew J.
25dcae6a-8289-4169-abb7-c45fff0bafdc
Kantawong, Fahsai, Burgess, Karl E.V., Jayawardena, Kamburapola, Hart, Andrew, Burchmore, Richard J., Gadegaard, Nikolaj, Oreffo, Richard O.C. and Dalby, Matthew J.
(2009)
Whole proteome analysis of osteoprogenitor differentiation induced by disordered nanotopography and mediated by ERK signalling.
Biomaterials, 30 (27), .
(doi:10.1016/j.biomaterials.2009.05.040).
(PMID:19560200)
Abstract
Topographic features can modulate cell behaviours such as proliferation, migration, differentiation and apoptosis. Biochemical mechanotransduction implies the conversion of mechanical forces (e.g. changes in cell spreading and morphology from changing surface topography) into biochemical signal via biomolecules. Still, little is known concerning which pathways may be directly involved in cell response to changes in the material surface. A number of pathways have been implicated using focused studies of ‘selected’ biomolecules rather than a global analysis of signal pathways. This study used a controlled disorder nanopit topography (NSQ50, fabricated by electron beam lithography) to direct osteoblast differentiation of progenitor cells. This topography is unique as it represents a middle route (from absolute order or random roughness) that allows osteoconversion with similar efficiency as dexamethasone and ascorbate treatment. Two direct-comparison proteomics techniques, firstly gel-based and then chromatography-based, were used to analyse progenitor proteome changes in response to the nanotopography. Many of the changed proteins form part of the Extracellular Signal-regulated Kinase (ERK1/2) pathway.
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Published date: September 2009
Keywords:
tissue engineering, disordered topography, extracellular-activated protein kinase, osteoprogenitor cells, proteomics
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Local EPrints ID: 181673
URI: http://eprints.soton.ac.uk/id/eprint/181673
ISSN: 0142-9612
PURE UUID: ee16fd79-f829-4191-981a-7db88b85add3
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Date deposited: 18 Apr 2011 08:55
Last modified: 15 Mar 2024 03:03
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Author:
Fahsai Kantawong
Author:
Karl E.V. Burgess
Author:
Kamburapola Jayawardena
Author:
Andrew Hart
Author:
Richard J. Burchmore
Author:
Nikolaj Gadegaard
Author:
Matthew J. Dalby
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