Hyperexpression of the high-affinity IgE receptor-beta chain in chronic allergic keratoconjunctivitis
Hyperexpression of the high-affinity IgE receptor-beta chain in chronic allergic keratoconjunctivitis
Purpose. Although the existence of Fc?RI-???2 and Fc?RI-??2 receptor subtypes was reported, there has been no direct evidence of these two subtypes of Fc?RI in vivo. To investigate the existence of these two subtypes of Fc?RI in vivo, the authors evaluated the expression of Fc?RI-? in the giant papillae of chronic allergic conjunctivitis and compared the expression level of Fc?RI-? with control conjunctivae using the anti–human Fc?RI-? antibody.
Methods. Fc?RI-? expression in giant papillae obtained from patients with atopic keratoconjunctivitis and vernal keratoconjunctivitis in control conjunctivae was evaluated by immunohistochemistry using anti–Fc?RI-?, -?, -?, and anti–human mast cell tryptase, anti–chymase, anti–basophil, and anti–CD1a antibodies.
Results. Statistical analyses revealed that the densities of Fc?RI-?+ cells, Fc?RI-?+ cells, tryptase+ cells, and Fc?RI-?+/tryptase+ cells were significantly increased in giant papillae compared with controls. There were two types of Fc?RI (???2 and ??2) on the mast cells of the giant papillae. The ratio of the Fc?RI-?+ cell number/Fc?RI-?+ cell number in the giant papillae (0.69 ± 0.08 [mean ± SD]) was significantly higher than that of the controls (0.07 ± 0.16). Fc?RI-?/tryptase double immunostaining revealed that 81% ± 13% of tryptase+ cells expressed Fc?RI-?. Fc?RI-?+ cells were preferentially localized within and around epithelial tissue. The authors also found that Fc?RI-? was expressed by basophils but not by Fc?RI-??2–positive Langerhans cells in the giant papillae samples.
Conclusions. Preferential Fc?RI-? expression observed in the mast cells and basophils of giant papillae suggests important roles of Fc?RI-? in the pathophysiology of atopic keratoconjunctivitis and vernal keratoconjunctivitis.
2871-2877
Matsuda, Akira
bd7d47ec-6e61-41aa-a3d1-ec30915821ed
Okayama, Yoshimichi
072adc30-6081-4d59-97a7-63a2a8ca5c87
Ebihara, Nobuyuki
4586996b-f68e-4319-a2eb-2327c5bf9a99
Yokoi, Norihiko
ddadff2c-cc95-49d6-b96a-d9d9c6eb3ac7
Hamuro, Junji
f94e2b95-320f-4543-90c3-68f695b42ae6
Walls, Andrew F.
aaa7e455-0562-4b4c-94f5-ec29c74b1bfe
Ra, Chisei
fa9ef555-9780-481f-9b44-589c074863d2
Hopkin, Julian M.
9ef25db9-a954-4288-a2bf-327009626a9b
Kinoshita, Shigeru
52d9806e-d186-4751-bfef-3b3c5cc0a331
June 2009
Matsuda, Akira
bd7d47ec-6e61-41aa-a3d1-ec30915821ed
Okayama, Yoshimichi
072adc30-6081-4d59-97a7-63a2a8ca5c87
Ebihara, Nobuyuki
4586996b-f68e-4319-a2eb-2327c5bf9a99
Yokoi, Norihiko
ddadff2c-cc95-49d6-b96a-d9d9c6eb3ac7
Hamuro, Junji
f94e2b95-320f-4543-90c3-68f695b42ae6
Walls, Andrew F.
aaa7e455-0562-4b4c-94f5-ec29c74b1bfe
Ra, Chisei
fa9ef555-9780-481f-9b44-589c074863d2
Hopkin, Julian M.
9ef25db9-a954-4288-a2bf-327009626a9b
Kinoshita, Shigeru
52d9806e-d186-4751-bfef-3b3c5cc0a331
Matsuda, Akira, Okayama, Yoshimichi, Ebihara, Nobuyuki, Yokoi, Norihiko, Hamuro, Junji, Walls, Andrew F., Ra, Chisei, Hopkin, Julian M. and Kinoshita, Shigeru
(2009)
Hyperexpression of the high-affinity IgE receptor-beta chain in chronic allergic keratoconjunctivitis.
Investigative Ophthalmology & Visual Science, 50 (6), .
(doi:10.1167/iovs.08-3022).
(PMID:19136704)
Abstract
Purpose. Although the existence of Fc?RI-???2 and Fc?RI-??2 receptor subtypes was reported, there has been no direct evidence of these two subtypes of Fc?RI in vivo. To investigate the existence of these two subtypes of Fc?RI in vivo, the authors evaluated the expression of Fc?RI-? in the giant papillae of chronic allergic conjunctivitis and compared the expression level of Fc?RI-? with control conjunctivae using the anti–human Fc?RI-? antibody.
Methods. Fc?RI-? expression in giant papillae obtained from patients with atopic keratoconjunctivitis and vernal keratoconjunctivitis in control conjunctivae was evaluated by immunohistochemistry using anti–Fc?RI-?, -?, -?, and anti–human mast cell tryptase, anti–chymase, anti–basophil, and anti–CD1a antibodies.
Results. Statistical analyses revealed that the densities of Fc?RI-?+ cells, Fc?RI-?+ cells, tryptase+ cells, and Fc?RI-?+/tryptase+ cells were significantly increased in giant papillae compared with controls. There were two types of Fc?RI (???2 and ??2) on the mast cells of the giant papillae. The ratio of the Fc?RI-?+ cell number/Fc?RI-?+ cell number in the giant papillae (0.69 ± 0.08 [mean ± SD]) was significantly higher than that of the controls (0.07 ± 0.16). Fc?RI-?/tryptase double immunostaining revealed that 81% ± 13% of tryptase+ cells expressed Fc?RI-?. Fc?RI-?+ cells were preferentially localized within and around epithelial tissue. The authors also found that Fc?RI-? was expressed by basophils but not by Fc?RI-??2–positive Langerhans cells in the giant papillae samples.
Conclusions. Preferential Fc?RI-? expression observed in the mast cells and basophils of giant papillae suggests important roles of Fc?RI-? in the pathophysiology of atopic keratoconjunctivitis and vernal keratoconjunctivitis.
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Published date: June 2009
Organisations:
Infection Inflammation & Immunity
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Local EPrints ID: 183801
URI: http://eprints.soton.ac.uk/id/eprint/183801
ISSN: 0146-0404
PURE UUID: 78bb635c-2be8-42b2-834b-3d68dbf5deaf
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Date deposited: 04 May 2011 09:23
Last modified: 15 Mar 2024 02:39
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Author:
Akira Matsuda
Author:
Yoshimichi Okayama
Author:
Nobuyuki Ebihara
Author:
Norihiko Yokoi
Author:
Junji Hamuro
Author:
Chisei Ra
Author:
Julian M. Hopkin
Author:
Shigeru Kinoshita
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