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Transcriptome analyses and biofilm-forming characteristics of a clonal Pseudomonas aeruginosa from the cystic fibrosis lung

Transcriptome analyses and biofilm-forming characteristics of a clonal Pseudomonas aeruginosa from the cystic fibrosis lung
Transcriptome analyses and biofilm-forming characteristics of a clonal Pseudomonas aeruginosa from the cystic fibrosis lung
Transmissible Pseudomonas aeruginosa clones potentially pose a serious threat to cystic fibrosis (CF) patients. The AES-1 clone has been found to infect up to 40 % of patients in five CF centres in eastern Australia. Studies were carried out on clonal and non-clonal (NC) isolates from chronically infected CF patients, and the reference strain PAO1, to gain insight into the properties of AES-1. The transcriptomes of AES-1 and NC isolates, and of PAO1, grown planktonically and as a 72 h biofilm were compared using PAO1 microarrays. Microarray data were validated using real-time PCR. Overall, most differentially expressed genes were downregulated. AES-1 differentially expressed bacteriophage genes, novel motility genes, and virulence and quorum-sensing-related genes, compared with both PAO1 and NC. AES-1 but not NC biofilms significantly downregulated aerobic respiration genes compared with planktonic growth, suggesting enhanced anaerobic/microaerophilic growth by AES-1. Biofilm measurement showed that AES-1 formed significantly larger and thicker biofilms than NC or PAO1 isolates. This may be related to expression of the gene PA0729, encoding a biofilm-enhancing bacteriophage, identified by PCR in all AES-1 but few NC isolates (n=42). Links with the Liverpool epidemic strain included the presence of PA0729 and the absence of the bacteriophage gene cluster PA0632–PA0639. No common markers were found with the Manchester strain. No particular differentially expressed gene in AES-1 could definitively be ascribed a role in its infectivity, thus increasing the likelihood that AES-1 infectivity is multi-factorial and possibly involves novel genes. This study extends our understanding of the transcriptomic and genetic differences between clonal and NC strains of P. aeruginosa from CF lung.
0022-2615
1454-1465
Manos, Jim
f453fe32-d123-4ad7-a9f4-6fd452f54017
Arthur, Jonathan
80cf624d-c75c-43d1-b788-0f46120a9c35
Rose, Barbara
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Tingpej, Pholawat
5ac55b4a-fc09-43e1-a62f-f6c5a98c7fc4
Fung, Carina
e1b4a27d-5b0b-4f62-8abc-11506c96c64f
Curtis, Michelle
8903a98c-89da-427b-a52a-ffc2ad63df98
Webb, Jeremy S.
ec0a5c4e-86cc-4ae9-b390-7298f5d65f8d
Hu, Honghua
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Kjelleberg, Staffan
043b66b5-130c-42f2-99b3-ec3eecf3248e
Gorrell, Mark D.
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Bye, Peter
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Harbour, Colin
c664b6a6-d77d-4418-afd9-24c3bc08259c
Manos, Jim
f453fe32-d123-4ad7-a9f4-6fd452f54017
Arthur, Jonathan
80cf624d-c75c-43d1-b788-0f46120a9c35
Rose, Barbara
8a503f97-55f6-4a24-985f-61c7863a5e7b
Tingpej, Pholawat
5ac55b4a-fc09-43e1-a62f-f6c5a98c7fc4
Fung, Carina
e1b4a27d-5b0b-4f62-8abc-11506c96c64f
Curtis, Michelle
8903a98c-89da-427b-a52a-ffc2ad63df98
Webb, Jeremy S.
ec0a5c4e-86cc-4ae9-b390-7298f5d65f8d
Hu, Honghua
0e6b1640-091a-46d1-9c1f-bd612b8682b9
Kjelleberg, Staffan
043b66b5-130c-42f2-99b3-ec3eecf3248e
Gorrell, Mark D.
93d7a66d-5b37-4b25-8828-c1d2cdc580c5
Bye, Peter
7339f456-5447-4071-82b5-7fa22ceba898
Harbour, Colin
c664b6a6-d77d-4418-afd9-24c3bc08259c

Manos, Jim, Arthur, Jonathan, Rose, Barbara, Tingpej, Pholawat, Fung, Carina, Curtis, Michelle, Webb, Jeremy S., Hu, Honghua, Kjelleberg, Staffan, Gorrell, Mark D., Bye, Peter and Harbour, Colin (2008) Transcriptome analyses and biofilm-forming characteristics of a clonal Pseudomonas aeruginosa from the cystic fibrosis lung. Journal of Medical Microbiology, 57 (12), 1454-1465. (doi:10.1099/jmm.0.2008/005009-0). (PMID:19018014)

Record type: Article

Abstract

Transmissible Pseudomonas aeruginosa clones potentially pose a serious threat to cystic fibrosis (CF) patients. The AES-1 clone has been found to infect up to 40 % of patients in five CF centres in eastern Australia. Studies were carried out on clonal and non-clonal (NC) isolates from chronically infected CF patients, and the reference strain PAO1, to gain insight into the properties of AES-1. The transcriptomes of AES-1 and NC isolates, and of PAO1, grown planktonically and as a 72 h biofilm were compared using PAO1 microarrays. Microarray data were validated using real-time PCR. Overall, most differentially expressed genes were downregulated. AES-1 differentially expressed bacteriophage genes, novel motility genes, and virulence and quorum-sensing-related genes, compared with both PAO1 and NC. AES-1 but not NC biofilms significantly downregulated aerobic respiration genes compared with planktonic growth, suggesting enhanced anaerobic/microaerophilic growth by AES-1. Biofilm measurement showed that AES-1 formed significantly larger and thicker biofilms than NC or PAO1 isolates. This may be related to expression of the gene PA0729, encoding a biofilm-enhancing bacteriophage, identified by PCR in all AES-1 but few NC isolates (n=42). Links with the Liverpool epidemic strain included the presence of PA0729 and the absence of the bacteriophage gene cluster PA0632–PA0639. No common markers were found with the Manchester strain. No particular differentially expressed gene in AES-1 could definitively be ascribed a role in its infectivity, thus increasing the likelihood that AES-1 infectivity is multi-factorial and possibly involves novel genes. This study extends our understanding of the transcriptomic and genetic differences between clonal and NC strains of P. aeruginosa from CF lung.

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Published date: 2008

Identifiers

Local EPrints ID: 186801
URI: http://eprints.soton.ac.uk/id/eprint/186801
ISSN: 0022-2615
PURE UUID: deaef254-48d0-4c56-b92e-b90cee9c00a1
ORCID for Jeremy S. Webb: ORCID iD orcid.org/0000-0003-2068-8589

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Date deposited: 16 May 2011 09:59
Last modified: 15 Mar 2024 03:26

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Contributors

Author: Jim Manos
Author: Jonathan Arthur
Author: Barbara Rose
Author: Pholawat Tingpej
Author: Carina Fung
Author: Michelle Curtis
Author: Jeremy S. Webb ORCID iD
Author: Honghua Hu
Author: Staffan Kjelleberg
Author: Mark D. Gorrell
Author: Peter Bye
Author: Colin Harbour

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