Windows to cell function and dysfunction: signatures written in the boundary layers
Windows to cell function and dysfunction: signatures written in the boundary layers
The medium surrounding cells either in culture or in tissues contains a chemical mix varying with cell state. As solutes move in and out of the cytoplasmic compartment they set up characteristic signatures in the cellular boundary layers. These layers are complex physical and chemical environments the profiles of which reflect cell physiology and provide conduits for intercellular messaging. Here we review some of the most relevant characteristics of the extracellular/intercellular space. Our initial focus is primarily on cultured cells but we extend our consideration to the far more complex environment of tissues, and discuss how chemical signatures in the boundary layer can or may affect cell function. Critical to the entire essay are the methods used, or being developed, to monitor chemical profiles in the boundary layers. We review recent developments in ultramicro electrochemical sensors and tailored optical reporters suitable for the task in hand.
chemical profiles, electrochemical sensing, imaging, intercellular space, unstirred layers
514-523
Smith, Peter J.S.
003de469-9420-4f12-8f0e-8e8d76d28d6c
Collis, Leon P.
66f825a3-f4a7-4e85-a1ca-ee16497b8a81
Messerli, Mark A.
94a8bd34-95d7-4a52-b4d8-1ccded0e8fe7
June 2010
Smith, Peter J.S.
003de469-9420-4f12-8f0e-8e8d76d28d6c
Collis, Leon P.
66f825a3-f4a7-4e85-a1ca-ee16497b8a81
Messerli, Mark A.
94a8bd34-95d7-4a52-b4d8-1ccded0e8fe7
Smith, Peter J.S., Collis, Leon P. and Messerli, Mark A.
(2010)
Windows to cell function and dysfunction: signatures written in the boundary layers.
BioEssays, 32 (6), .
(doi:10.1002/bies.200900173).
(PMID:20486138)
Abstract
The medium surrounding cells either in culture or in tissues contains a chemical mix varying with cell state. As solutes move in and out of the cytoplasmic compartment they set up characteristic signatures in the cellular boundary layers. These layers are complex physical and chemical environments the profiles of which reflect cell physiology and provide conduits for intercellular messaging. Here we review some of the most relevant characteristics of the extracellular/intercellular space. Our initial focus is primarily on cultured cells but we extend our consideration to the far more complex environment of tissues, and discuss how chemical signatures in the boundary layer can or may affect cell function. Critical to the entire essay are the methods used, or being developed, to monitor chemical profiles in the boundary layers. We review recent developments in ultramicro electrochemical sensors and tailored optical reporters suitable for the task in hand.
Text
BioEssays_Review_final012610_inc_figs.pdf
- Author's Original
More information
e-pub ahead of print date: 18 May 2010
Published date: June 2010
Keywords:
chemical profiles, electrochemical sensing, imaging, intercellular space, unstirred layers
Organisations:
Centre for Biological Sciences
Identifiers
Local EPrints ID: 188743
URI: http://eprints.soton.ac.uk/id/eprint/188743
ISSN: 0265-9247
PURE UUID: e3dcda47-b730-43f7-b5a6-a87fd16a4f64
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Date deposited: 27 May 2011 15:58
Last modified: 15 Mar 2024 03:38
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Contributors
Author:
Leon P. Collis
Author:
Mark A. Messerli
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