The University of Southampton
University of Southampton Institutional Repository

Scattering attenuation microscopy of oral epithelial dysplasia

Scattering attenuation microscopy of oral epithelial dysplasia
Scattering attenuation microscopy of oral epithelial dysplasia
We present a new method for quantitative visualization of premalignant oral epithelium called scattering attenuation microscopy (SAM). Using low-coherence interferometry, SAM projects measurements of epithelial optical attenuation onto an image of the tissue surface as a color map. The measured attenuation is dominated by optical scattering that provides a metric of the severity of oral epithelial dysplasia (OED). Scattering is sensitive to the changes in size and distribution of nuclear material that are characteristic of OED, a condition recognized by the occurrence of basal-cell-like features throughout the epithelial depth. SAM measures the axial intensity change of light backscattered from epithelial tissue. Scattering measurements are obtained from sequential axial scans of a 3-D tissue volume and displayed as a 2-D SAM image. A novel segmentation method is used to confine scattering measurement to epithelial tissue. This is applied to oral biopsy samples obtained from 19 patients. Our results show that imaging of tissue scattering can be used to discriminate between different dysplastic severities and furthermore presents a powerful tool for identifying the most representative tissue site for biopsy
1083-3668
066003-[8pp]
Tomlins, Pete H.
0a610b75-354a-4ee6-bb0d-3eb2a9540680
Adegun, Oluyori
758ae442-f7f3-45e8-8e32-811348d56781
Hagi-Pavli, Eleni
92d0f148-9c96-4d14-8662-512cc70bd145
Piper, Kim
74470819-f897-4052-acd7-309c89f31cc1
Fortune, Farida
9fd3d817-1fb6-4abb-b5aa-ca96c26df159
Bader, Dan
9884d4f6-2607-4d48-bf0c-62bdcc0d1dbf
Tomlins, Pete H.
0a610b75-354a-4ee6-bb0d-3eb2a9540680
Adegun, Oluyori
758ae442-f7f3-45e8-8e32-811348d56781
Hagi-Pavli, Eleni
92d0f148-9c96-4d14-8662-512cc70bd145
Piper, Kim
74470819-f897-4052-acd7-309c89f31cc1
Fortune, Farida
9fd3d817-1fb6-4abb-b5aa-ca96c26df159
Bader, Dan
9884d4f6-2607-4d48-bf0c-62bdcc0d1dbf

Tomlins, Pete H., Adegun, Oluyori, Hagi-Pavli, Eleni, Piper, Kim, Fortune, Farida and Bader, Dan (2010) Scattering attenuation microscopy of oral epithelial dysplasia. Journal of Biomedical Optics, 15, 066003-[8pp]. (doi:10.1117/1.3505019). (PMID:21198177)

Record type: Article

Abstract

We present a new method for quantitative visualization of premalignant oral epithelium called scattering attenuation microscopy (SAM). Using low-coherence interferometry, SAM projects measurements of epithelial optical attenuation onto an image of the tissue surface as a color map. The measured attenuation is dominated by optical scattering that provides a metric of the severity of oral epithelial dysplasia (OED). Scattering is sensitive to the changes in size and distribution of nuclear material that are characteristic of OED, a condition recognized by the occurrence of basal-cell-like features throughout the epithelial depth. SAM measures the axial intensity change of light backscattered from epithelial tissue. Scattering measurements are obtained from sequential axial scans of a 3-D tissue volume and displayed as a 2-D SAM image. A novel segmentation method is used to confine scattering measurement to epithelial tissue. This is applied to oral biopsy samples obtained from 19 patients. Our results show that imaging of tissue scattering can be used to discriminate between different dysplastic severities and furthermore presents a powerful tool for identifying the most representative tissue site for biopsy

Text
adegun_JBOPFO_2010.pdf - Other
Restricted to Repository staff only
Request a copy

More information

Published date: December 2010

Identifiers

Local EPrints ID: 189253
URI: http://eprints.soton.ac.uk/id/eprint/189253
ISSN: 1083-3668
PURE UUID: 3e21f600-74ab-4d30-981e-f06170d3d584
ORCID for Dan Bader: ORCID iD orcid.org/0000-0002-1208-3507

Catalogue record

Date deposited: 02 Jun 2011 14:31
Last modified: 14 Mar 2024 03:35

Export record

Altmetrics

Contributors

Author: Pete H. Tomlins
Author: Oluyori Adegun
Author: Eleni Hagi-Pavli
Author: Kim Piper
Author: Farida Fortune
Author: Dan Bader ORCID iD

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×