Scattering attenuation microscopy of oral epithelial dysplasia
Scattering attenuation microscopy of oral epithelial dysplasia
We present a new method for quantitative visualization of premalignant oral epithelium called scattering attenuation microscopy (SAM). Using low-coherence interferometry, SAM projects measurements of epithelial optical attenuation onto an image of the tissue surface as a color map. The measured attenuation is dominated by optical scattering that provides a metric of the severity of oral epithelial dysplasia (OED). Scattering is sensitive to the changes in size and distribution of nuclear material that are characteristic of OED, a condition recognized by the occurrence of basal-cell-like features throughout the epithelial depth. SAM measures the axial intensity change of light backscattered from epithelial tissue. Scattering measurements are obtained from sequential axial scans of a 3-D tissue volume and displayed as a 2-D SAM image. A novel segmentation method is used to confine scattering measurement to epithelial tissue. This is applied to oral biopsy samples obtained from 19 patients. Our results show that imaging of tissue scattering can be used to discriminate between different dysplastic severities and furthermore presents a powerful tool for identifying the most representative tissue site for biopsy
066003-[8pp]
Tomlins, Pete H.
0a610b75-354a-4ee6-bb0d-3eb2a9540680
Adegun, Oluyori
758ae442-f7f3-45e8-8e32-811348d56781
Hagi-Pavli, Eleni
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Piper, Kim
74470819-f897-4052-acd7-309c89f31cc1
Fortune, Farida
9fd3d817-1fb6-4abb-b5aa-ca96c26df159
Bader, Dan
9884d4f6-2607-4d48-bf0c-62bdcc0d1dbf
December 2010
Tomlins, Pete H.
0a610b75-354a-4ee6-bb0d-3eb2a9540680
Adegun, Oluyori
758ae442-f7f3-45e8-8e32-811348d56781
Hagi-Pavli, Eleni
92d0f148-9c96-4d14-8662-512cc70bd145
Piper, Kim
74470819-f897-4052-acd7-309c89f31cc1
Fortune, Farida
9fd3d817-1fb6-4abb-b5aa-ca96c26df159
Bader, Dan
9884d4f6-2607-4d48-bf0c-62bdcc0d1dbf
Tomlins, Pete H., Adegun, Oluyori, Hagi-Pavli, Eleni, Piper, Kim, Fortune, Farida and Bader, Dan
(2010)
Scattering attenuation microscopy of oral epithelial dysplasia.
Journal of Biomedical Optics, 15, .
(doi:10.1117/1.3505019).
(PMID:21198177)
Abstract
We present a new method for quantitative visualization of premalignant oral epithelium called scattering attenuation microscopy (SAM). Using low-coherence interferometry, SAM projects measurements of epithelial optical attenuation onto an image of the tissue surface as a color map. The measured attenuation is dominated by optical scattering that provides a metric of the severity of oral epithelial dysplasia (OED). Scattering is sensitive to the changes in size and distribution of nuclear material that are characteristic of OED, a condition recognized by the occurrence of basal-cell-like features throughout the epithelial depth. SAM measures the axial intensity change of light backscattered from epithelial tissue. Scattering measurements are obtained from sequential axial scans of a 3-D tissue volume and displayed as a 2-D SAM image. A novel segmentation method is used to confine scattering measurement to epithelial tissue. This is applied to oral biopsy samples obtained from 19 patients. Our results show that imaging of tissue scattering can be used to discriminate between different dysplastic severities and furthermore presents a powerful tool for identifying the most representative tissue site for biopsy
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Published date: December 2010
Identifiers
Local EPrints ID: 189253
URI: http://eprints.soton.ac.uk/id/eprint/189253
ISSN: 1083-3668
PURE UUID: 3e21f600-74ab-4d30-981e-f06170d3d584
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Date deposited: 02 Jun 2011 14:31
Last modified: 14 Mar 2024 03:35
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Author:
Pete H. Tomlins
Author:
Oluyori Adegun
Author:
Eleni Hagi-Pavli
Author:
Kim Piper
Author:
Farida Fortune
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