Recognition of GT mismatches by Vsr mismatch endonuclease
Fox, K. R., Allinson, S. L., Sahagun-Krause, H. and Brown, T. (2000) Recognition of GT mismatches by Vsr mismatch endonuclease. Nucleic Acids Research, 28, (13), 2535-2540. (doi:10.1093/nar/28.13.2535).
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Description/Abstract
The Vsr mismatch endonuclease recognises the sequence CIWGG (W = A or T) in which the underlined thymine is paired with guanine and nicks the DNA backbone on the 5'-side of the mispaired thymine, By using base analogues of G and T we have explored the functional groups on the mismatch pair which are recognised by the enzyme. Removal of the thymine 5-methyl group causes a 60% reduction in activity, while removing the 2-amino group of guanine reduces cleavage by 90%, Placing 2-aminopurine or nebularine opposite T generates mismatches which are cut at a much lower rate (0.1%), When either base is removed, generating a pseudoabasic site (1',2'-dideoxyribose), the enzyme still produces site-specific cleavage, but at only 1% of the original rate. Although TT and CT mismatches at this position are cleaved at a low rate (similar to 1%), mismatches with other bases (such as GA and AC) and Watson-Crick base pairs are not cleaved by the enzyme. There is also no cleavage when the mismatched T is replaced with difluorotoluene.
| Item Type: | Article |
|---|---|
| Related URLs: | |
| Keywords: | escherichia-coli k-12, short patch repair, cytosine methylation, crystal-structure, dna duplex, gene, 5-methyl-cytosine, difluorotoluene, cleavage, adenine |
| Subjects: | Q Science Q Science > QD Chemistry |
| Divisions: | University Structure - Pre August 2011 > School of Chemistry |
| Item ID: | 18926 |
| Date Deposited: | 19 Jan 2006 |
| Last Modified: | 01 Jun 2011 01:46 |
| Contributors: | Fox, K. R. (Author) Allinson, S. L. (Author) Sahagun-Krause, H. (Author) Brown, T. (Author) |
| Date: | 1 July 2000 |
| Status: | Published |
| URI: | http://eprints.soton.ac.uk/id/eprint/18926 |
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