Enzymatic and immunologic identification of succinic semialdehyde dehydrogenase in rat and human neural and nonneural tissues


Chambliss, Ken L., Zhang, Yu-An, Rossier, Eva, Vollmer, Brigitte and Gibson, K. Michael (1995) Enzymatic and immunologic identification of succinic semialdehyde dehydrogenase in rat and human neural and nonneural tissues. Journal of Neurochemistry, 65, (2), 851-855. (doi:10.1046/j.1471-4159.1995.65020851.x). (PMID:7616245).

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Description/Abstract

We have identified succinic semialdehyde dehydrogenase protein in rat and human neural and nonneural tissues. Tissue localization was determined by enzymatic assay and by western immunoblotting using polyclonal antibodies raised in rabbit against the purified rat brain protein. Although brain shows the highest level of succinic semialdehyde dehydrogenase activity, substantial amounts of enzyme activity occur in mammalian liver, pituitary, heart, and ovary. We further demonstrate the absence of succinic semialdehyde dehydrogenase enzyme activity and protein in brain, liver, and kidney tissue samples from an individual affected with succinic semialdehyde dehydrogenase deficiency, thereby verifying the specificity of our antibodies.

Item Type: Article
ISSNs: 0022-3042 (print)
1471-4159 (electronic)
Keywords: succinic semialdehyde dehydrogenase, neural tissue, nonneural tissue, spectrophotometry, immunoblot, inborn error of metabolism
Subjects: Q Science > QD Chemistry
R Medicine > RC Internal medicine > RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry
Divisions: University Structure - Pre August 2011 > School of Medicine > Clinical Neurosciences
ePrint ID: 189531
Date Deposited: 02 Jun 2011 15:02
Last Modified: 27 Mar 2014 19:42
URI: http://eprints.soton.ac.uk/id/eprint/189531

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