Enzymatic and immunologic identification of succinic semialdehyde dehydrogenase in rat and human neural and nonneural tissues
Chambliss, Ken L., Zhang, Yu-An, Rossier, Eva, Vollmer, Brigitte and Gibson, K. Michael (1995) Enzymatic and immunologic identification of succinic semialdehyde dehydrogenase in rat and human neural and nonneural tissues. Journal of Neurochemistry, 65, (2), 851-855. (doi:10.1046/j.1471-4159.1995.65020851.x). (PMID:7616245).
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We have identified succinic semialdehyde dehydrogenase protein in rat and human neural and nonneural tissues. Tissue localization was determined by enzymatic assay and by western immunoblotting using polyclonal antibodies raised in rabbit against the purified rat brain protein. Although brain shows the highest level of succinic semialdehyde dehydrogenase activity, substantial amounts of enzyme activity occur in mammalian liver, pituitary, heart, and ovary. We further demonstrate the absence of succinic semialdehyde dehydrogenase enzyme activity and protein in brain, liver, and kidney tissue samples from an individual affected with succinic semialdehyde dehydrogenase deficiency, thereby verifying the specificity of our antibodies.
|Keywords:||succinic semialdehyde dehydrogenase, neural tissue, nonneural tissue, spectrophotometry, immunoblot, inborn error of metabolism|
|Subjects:||Q Science > QD Chemistry
R Medicine > RC Internal medicine > RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry
|Divisions:||University Structure - Pre August 2011 > School of Medicine > Clinical Neurosciences
|Date Deposited:||02 Jun 2011 15:02|
|Last Modified:||02 Mar 2012 11:55|
|RDF:||RDF+N-Triples, RDF+N3, RDF+XML, Browse.|
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