The mechanism of DNA repair by uracil-DNA glycosylase: Studies using nucleotide analogues


Rosler, A., Panayotou, G., Hornby, D. P., Barlow, T., Brown, T., Pearl, L. H., Savva, R. and Blackburn, G. M. (2000) The mechanism of DNA repair by uracil-DNA glycosylase: Studies using nucleotide analogues. Nucleosides Nucleotides and Nucleic Acids, 19, (10-12), 1505-1516.

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Description/Abstract

2',4'-Dideoxy-4'-methyleneuridine incorporated into oligodeoxynucleotides forms regular B-DNA duplexes as shown by Tm and CD measurements. Such oligomers are not cleaved by the DNA repair enzyme, UDG, which cleaves the glycosylic bond in dU but not in dT nor in dC nucleosides in single stranded and double stranded DNA. Differential binding of oligomers containing carbadU, 4'-thiodU, and dU residues to wild type and mutant UDG proteins identify an essential role for the furanose 4'-oxygen in recognition and cleavage of dU residues in DNA.

Item Type: Article
Related URLs:
Keywords: escherichia-coli, cytosine residues, structural basis, excision, catalysis, cyclopentane, glycosidase, deamination, nucleosides, inhibitor
Subjects: Q Science
Q Science > QD Chemistry
Divisions: University Structure - Pre August 2011 > School of Chemistry
ePrint ID: 18977
Date Deposited: 18 Jan 2006
Last Modified: 27 Mar 2014 18:08
URI: http://eprints.soton.ac.uk/id/eprint/18977

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