The effect of three lipid emulsions differing in fatty acid composition on growth, apoptosis and cell cycle arrest in the HT-29 colorectal cancer cell line
The effect of three lipid emulsions differing in fatty acid composition on growth, apoptosis and cell cycle arrest in the HT-29 colorectal cancer cell line
Background & aims: An in vitro study showed that a lipid emulsion containing fish oil (FO) slows the growth of colon cancer cells and enhances their sensitivity to 5-fluorouracil (FU). The aim was to confirm this finding and to compare such an emulsion with an alternative to lowered n-6 fatty acid exposure.
Methods: We determined the number of viable cells, apoptosis and cell cycle distribution of HT-29 cells after exposure to one of three lipid emulsions. Cell cycle distribution was also assessed after treatment with lipid emulsions and FU.
Results: The lipid emulsion containing FO induced a significant growth inhibitory effect without changing the percentage of apoptotic cells. Exposure to the other lipid emulsions had no effect on growth and decreased apoptosis. Each lipid emulsion potentiated the S phase-halting effect of 1 and 10 ?M FU. This effect also occurred at 0.1 ?M FU when the cells were exposed to the FO containing lipid emulsion.
Conclusions: A lipid emulsion containing FO has a growth inhibitory effect on a human colon adenocarcinoma cell line, an effect not due to the induction of apoptosis, and potentiated the S phase-halting effect of FU. Thus, an FO lipid emulsion may be of benefit in colorectal cancer.
fish oil, lipid emulsion, cell growth, cell cycle, apoptosis, 5-fluorouracil
519-524
Sala-Vila, Aleix
d3ddb143-7c9b-44c0-b2ab-28a00fa9ac7e
Folkes, Jacqueline
7810a5d2-48d3-4e40-a64f-808c8f81a62d
Calder, Phillip C.
1797e54f-378e-4dcb-80a4-3e30018f07a6
August 2010
Sala-Vila, Aleix
d3ddb143-7c9b-44c0-b2ab-28a00fa9ac7e
Folkes, Jacqueline
7810a5d2-48d3-4e40-a64f-808c8f81a62d
Calder, Phillip C.
1797e54f-378e-4dcb-80a4-3e30018f07a6
Sala-Vila, Aleix, Folkes, Jacqueline and Calder, Phillip C.
(2010)
The effect of three lipid emulsions differing in fatty acid composition on growth, apoptosis and cell cycle arrest in the HT-29 colorectal cancer cell line.
Clinical Nutrition, 29 (4), .
(doi:10.1016/j.clnu.2009.11.004).
Abstract
Background & aims: An in vitro study showed that a lipid emulsion containing fish oil (FO) slows the growth of colon cancer cells and enhances their sensitivity to 5-fluorouracil (FU). The aim was to confirm this finding and to compare such an emulsion with an alternative to lowered n-6 fatty acid exposure.
Methods: We determined the number of viable cells, apoptosis and cell cycle distribution of HT-29 cells after exposure to one of three lipid emulsions. Cell cycle distribution was also assessed after treatment with lipid emulsions and FU.
Results: The lipid emulsion containing FO induced a significant growth inhibitory effect without changing the percentage of apoptotic cells. Exposure to the other lipid emulsions had no effect on growth and decreased apoptosis. Each lipid emulsion potentiated the S phase-halting effect of 1 and 10 ?M FU. This effect also occurred at 0.1 ?M FU when the cells were exposed to the FO containing lipid emulsion.
Conclusions: A lipid emulsion containing FO has a growth inhibitory effect on a human colon adenocarcinoma cell line, an effect not due to the induction of apoptosis, and potentiated the S phase-halting effect of FU. Thus, an FO lipid emulsion may be of benefit in colorectal cancer.
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Published date: August 2010
Keywords:
fish oil, lipid emulsion, cell growth, cell cycle, apoptosis, 5-fluorouracil
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Local EPrints ID: 190143
URI: http://eprints.soton.ac.uk/id/eprint/190143
ISSN: 0261-5614
PURE UUID: 11d67709-5e53-4cd1-9073-49e7422dcf7b
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Date deposited: 10 Jun 2011 08:13
Last modified: 15 Mar 2024 02:50
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Author:
Aleix Sala-Vila
Author:
Jacqueline Folkes
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