Long-term culture of fully differentiated adult insect neurons

Smith, Peter J.S. and Howes, Elizabeth A. (1996) Long-term culture of fully differentiated adult insect neurons. Journal of Neuroscience Methods, 69, (1), 113-122. (doi:10.1016/S0165-0270(96)00026-X).


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An identified group of cells, dorsal unpaired median (DUM) neurons, isolated from the central nervous system of adult cockroaches can be grown in vitro for extended periods. These cells often develop morphological characteristics that differ from their in situ appearance but physiological experiments demonstrate that they retain their distinctive in vivo membrane properties. A method for culturing insect CNS neurons, which includes the use of a haemolymph-derived growth-enhancing factor is described, and this technique is compared with other attempts to develop an efficient system for producing in vitro preparations of well-defined, identifiable neurons that would provide a model system for aspects of neuron developmental growth, repair and function.

Item Type: Article
ISSNs: 0165-0270 (print)
Keywords: periplaneta americana, cockroach dum neuron, growth factor, insect hemolymph
Subjects: Q Science > QH Natural history > QH301 Biology
R Medicine > RC Internal medicine > RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry
Divisions: University Structure - Pre August 2011 > Other
ePrint ID: 190543
Date Deposited: 15 Jun 2011 12:17
Last Modified: 27 Mar 2014 19:42
URI: http://eprints.soton.ac.uk/id/eprint/190543

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