Dietary free oleic and linoleic acid enhances neutrophil function and modulates the inflammatory response in rats
Dietary free oleic and linoleic acid enhances neutrophil function and modulates the inflammatory response in rats
The high ingestion of oleic (OLA) and linoleic (LNA) acids by Western populations, the presence of inflammatory diseases in these populations, and the importance of neutrophils in the inflammatory process led us to investigate the effects of oral ingestion of unesterified OLA and LNA on rat neutrophil function. Pure OLA and LNA were administered by gavage over 10 days. The doses used (0.11, 0.22 and 0.44 g/kg of body weight) were based on the Western consumption of OLA and LNA. Neither fatty acid affected food, calorie or water intake. The fatty acids were not toxic to neutrophils as evaluated by cytometry using propidium iodide (membrane integrity and DNA fragmentation). Neutrophil migration in response to intraperitoneal injection of glycogen and in the air pouch assay, was elevated after administration of either OLA or LNA. This effect was associated with enhancement of rolling and increased release of the chemokine CINC-2alphabeta. Both fatty acids elevated L-selectin expression, whereas no effect on beta(2)-integrin expression was observed, as evaluated by flow cytometry. LNA increased the production of proinflammatory cytokines (IL-1beta and CINC-2alphabeta) by neutrophils after 4 h in culture and both fatty acids decreased the release of the same cytokines after 18 h. In conclusion, OLA and LNA modulate several functions of neutrophils and can influence the inflammatory process
809-819
Rodrigues, Hosana Gomes
f28c7c9b-fd12-4979-933d-d5ce7c7a949d
Vinolo, Marco Aurelio
bc03379a-7394-45ae-9d39-3199109146b8
Magdalon, Juliana
27683b0c-d552-4276-bebf-94ba21d67071
Fujiwara, Harolodo
24fe39f2-9d9f-4209-93ab-00efcee8bd98
Cavalcanti, Danielle M.
08ab3e66-769b-4661-a3f9-450acb572847
Farsky, Sandra H. P.
ff35d9bc-551e-494e-9a70-4f8cf0779f58
Calder, Philip C.
1797e54f-378e-4dcb-80a4-3e30018f07a6
Hatanaka, Elaine
a75419b0-65c9-496b-ba2e-62e716fd0965
Curi, Rui
87abee54-a75d-4e94-b9b9-2169d8f97fec
September 2010
Rodrigues, Hosana Gomes
f28c7c9b-fd12-4979-933d-d5ce7c7a949d
Vinolo, Marco Aurelio
bc03379a-7394-45ae-9d39-3199109146b8
Magdalon, Juliana
27683b0c-d552-4276-bebf-94ba21d67071
Fujiwara, Harolodo
24fe39f2-9d9f-4209-93ab-00efcee8bd98
Cavalcanti, Danielle M.
08ab3e66-769b-4661-a3f9-450acb572847
Farsky, Sandra H. P.
ff35d9bc-551e-494e-9a70-4f8cf0779f58
Calder, Philip C.
1797e54f-378e-4dcb-80a4-3e30018f07a6
Hatanaka, Elaine
a75419b0-65c9-496b-ba2e-62e716fd0965
Curi, Rui
87abee54-a75d-4e94-b9b9-2169d8f97fec
Rodrigues, Hosana Gomes, Vinolo, Marco Aurelio, Magdalon, Juliana, Fujiwara, Harolodo, Cavalcanti, Danielle M., Farsky, Sandra H. P., Calder, Philip C., Hatanaka, Elaine and Curi, Rui
(2010)
Dietary free oleic and linoleic acid enhances neutrophil function and modulates the inflammatory response in rats.
Lipids, 45 (9), .
(doi:10.1007/s11745-010-3461-9).
(PMID:20730605)
Abstract
The high ingestion of oleic (OLA) and linoleic (LNA) acids by Western populations, the presence of inflammatory diseases in these populations, and the importance of neutrophils in the inflammatory process led us to investigate the effects of oral ingestion of unesterified OLA and LNA on rat neutrophil function. Pure OLA and LNA were administered by gavage over 10 days. The doses used (0.11, 0.22 and 0.44 g/kg of body weight) were based on the Western consumption of OLA and LNA. Neither fatty acid affected food, calorie or water intake. The fatty acids were not toxic to neutrophils as evaluated by cytometry using propidium iodide (membrane integrity and DNA fragmentation). Neutrophil migration in response to intraperitoneal injection of glycogen and in the air pouch assay, was elevated after administration of either OLA or LNA. This effect was associated with enhancement of rolling and increased release of the chemokine CINC-2alphabeta. Both fatty acids elevated L-selectin expression, whereas no effect on beta(2)-integrin expression was observed, as evaluated by flow cytometry. LNA increased the production of proinflammatory cytokines (IL-1beta and CINC-2alphabeta) by neutrophils after 4 h in culture and both fatty acids decreased the release of the same cytokines after 18 h. In conclusion, OLA and LNA modulate several functions of neutrophils and can influence the inflammatory process
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Published date: September 2010
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Local EPrints ID: 190881
URI: http://eprints.soton.ac.uk/id/eprint/190881
ISSN: 0024-4201
PURE UUID: aa06c6aa-85bc-46bf-99dd-cb399e1c2560
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Date deposited: 16 Jun 2011 10:25
Last modified: 15 Mar 2024 02:50
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Author:
Hosana Gomes Rodrigues
Author:
Marco Aurelio Vinolo
Author:
Juliana Magdalon
Author:
Harolodo Fujiwara
Author:
Danielle M. Cavalcanti
Author:
Sandra H. P. Farsky
Author:
Elaine Hatanaka
Author:
Rui Curi
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