β-Hydroxy-β-methylbutyrate modifies human peripheral blood mononuclear cell prliferation and cytokine production in vitro
Nunes, Everson A., Lomax , Amy R., Noakes , Paul S., Miles, Elizabeth A., Fernandes, Luiz C. and Calder, Phillip C. (2011) β-Hydroxy-β-methylbutyrate modifies human peripheral blood mononuclear cell prliferation and cytokine production in vitro. Nutrition, 27, (1), 92-99. (doi:10.1016/j.nut.2009.12.008)
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Description/Abstract
Above 1 mM, HMB decreased the extent of proliferation normally observed after stimulation by concanavalin A. The decrease was evident at 10 mM HMB, when the proliferation index was 50% reduced when compared with the absence of HMB. Cell cycle analysis demonstrated an increase in the proportion of cells at the G0-G1 phase at 10 mM HMB. CD25 and pERK1/2 expression were not related to the observed effect on proliferation. HMB affected the concentrations of all five cytokines measured following stimulation. Tumor necrosis factor-α concentration in the culture medium was reduced by 35% at all HMB concentrations. Th1/Th2 cytokine production was modified toward a Th2 profile when HMB was at 1 or 10 mM. Thus, HMB at 10 mM impairs lymphocyte proliferation and progression through the cell cycle. The lowest concentration used here (0.1 mM) exerted some actions on cytokine production, including decreasing TNF-α production, but not on proliferation and cell cycle progression.
| Item Type: | Article |
|---|---|
| ISSN: | 0899-9007 (print) |
| Uncontrolled Keywords: | hmb, cytokines, lymphocyte, proliferation, immunomodulation, cell cycle |
| Subjects: | Q Science > QR Microbiology > QR180 Immunology R Medicine > RB Pathology R Medicine > RC Internal medicine > RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry |
| Divisions: | University Structure - Pre August 2011 > School of Medicine > Developmental Origins of Health and Disease |
| ePrint ID: | 192109 |
| URI: | http://eprints.soton.ac.uk/id/eprint/192109 |
| Deposited On: | 29 Jun 2011 15:52 |
| Last Modified: | 25 Apr 2012 08:50 |
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