A novel fluorescent sensitive assay for detection of differential T cell mediated lysis of multiple adherent target cells
Black, Antony P., Jones, Louise, Ardern-Jones, Michael and Ogg, Graham S. (2006) A novel fluorescent sensitive assay for detection of differential T cell mediated lysis of multiple adherent target cells. Journal of Immunological Methods, 316, (1-2), 153-157. (doi:10.1016/j.jim.2006.07.022). (PMID:16989854).
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Description/Abstract
There are few studies that have investigated T cell mediated lysis of adherent cells. We have developed a novel, rapid and sensitive fluorescent dye-swap assay that allows efficient detection of adherent target cell lysis. The assay allows simultaneous use of multiple differentially sensitised targets and facilitates concomitant surface or intracellular effector cell phenotypic analysis.
| Item Type: | Article |
|---|---|
| ISSNs: | 0022-1759 (print) |
| Keywords: | killing, cytotoxicity, T cell, adherent |
| Subjects: | Q Science > QR Microbiology > QR180 Immunology R Medicine > R Medicine (General) |
| Divisions: | University Structure - Pre August 2011 > School of Medicine > Infection, Inflammation and Repair |
| Item ID: | 192729 |
| Date Deposited: | 07 Jul 2011 12:29 |
| Last Modified: | 07 Jul 2011 12:29 |
| Contributors: | Black, Antony P. (Author) Jones, Louise (Author) Ardern-Jones, Michael (Author) Ogg, Graham S. (Author) |
| Date: | 30 October 2006 |
| Status: | Published |
| URI: | http://eprints.soton.ac.uk/id/eprint/192729 |
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