A novel fluorescent sensitive assay for detection of differential T cell mediated lysis of multiple adherent target cells
A novel fluorescent sensitive assay for detection of differential T cell mediated lysis of multiple adherent target cells
There are few studies that have investigated T cell mediated lysis of adherent cells. We have developed a novel, rapid and sensitive fluorescent dye-swap assay that allows efficient detection of adherent target cell lysis. The assay allows simultaneous use of multiple differentially sensitised targets and facilitates concomitant surface or intracellular effector cell phenotypic analysis.
killing, cytotoxicity, T cell, adherent
153-157
Black, Antony P.
524b404d-014d-4d98-a3ad-6a73172c3b1a
Jones, Louise
68ce5626-a7bb-4856-93f3-77502a235d5e
Ardern-Jones, Michael
7ac43c24-94ab-4d19-ba69-afaa546bec90
Ogg, Graham S.
eabec6c9-1ef9-4ca6-b985-25f00a4ab358
30 October 2006
Black, Antony P.
524b404d-014d-4d98-a3ad-6a73172c3b1a
Jones, Louise
68ce5626-a7bb-4856-93f3-77502a235d5e
Ardern-Jones, Michael
7ac43c24-94ab-4d19-ba69-afaa546bec90
Ogg, Graham S.
eabec6c9-1ef9-4ca6-b985-25f00a4ab358
Black, Antony P., Jones, Louise, Ardern-Jones, Michael and Ogg, Graham S.
(2006)
A novel fluorescent sensitive assay for detection of differential T cell mediated lysis of multiple adherent target cells.
Journal of Immunological Methods, 316 (1-2), .
(doi:10.1016/j.jim.2006.07.022).
(PMID:16989854)
Abstract
There are few studies that have investigated T cell mediated lysis of adherent cells. We have developed a novel, rapid and sensitive fluorescent dye-swap assay that allows efficient detection of adherent target cell lysis. The assay allows simultaneous use of multiple differentially sensitised targets and facilitates concomitant surface or intracellular effector cell phenotypic analysis.
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Published date: 30 October 2006
Keywords:
killing, cytotoxicity, T cell, adherent
Identifiers
Local EPrints ID: 192729
URI: http://eprints.soton.ac.uk/id/eprint/192729
ISSN: 0022-1759
PURE UUID: f5da7bbc-0a0f-43ee-94f7-341f1c2b485c
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Date deposited: 07 Jul 2011 12:29
Last modified: 15 Mar 2024 03:28
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Contributors
Author:
Antony P. Black
Author:
Louise Jones
Author:
Graham S. Ogg
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