Enzyme-linked immunosorbent assay based on recombinant human group C rotavirus inner capsid protein (VP6) To detect human group C rotaviruses in fecal samples
James, V.L.A., Lambden, P.R., Caul, E.O. and Clarke, I.N. (1998) Enzyme-linked immunosorbent assay based on recombinant human group C rotavirus inner capsid protein (VP6) To detect human group C rotaviruses in fecal samples. Journal of Clinical Microbiology, 36, (11), 3178-3181. (PMID:9431910).
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Description/Abstract
A recent study showed that 43% of a population in the United Kingdom were seropositive for group C rotavirus. The higher than expected incidence may be due to limited diagnosis of acute human group C rotavirus infections because no routine test is available. Human group C rotavirus infections are routinely diagnosed by electron microscopy (EM) and a negative group A rotavirus enzyme-linked immunosorbent assay (ELISA) result. An antigen-detection ELISA was developed with hyperimmune antibodies raised to human group C rotavirus recombinant VP6 (Bristol strain) expressed in insect cells. The assay was used to screen fecal samples to determine the prevalence of group C rotavirus infection. Samples positive by ELISA were confirmed by EM, polyacrylamide gel electrophoresis of double-stranded RNA, or detection of the VP6 gene by reverse transcription-PCR. Retrospective analysis indicated a 1 to 2% detection rate of positivity among samples from patients with acute diarrhea.
| Item Type: | Article |
|---|---|
| ISSNs: | 0095-1137 (print) |
| Related URLs: | |
| Subjects: | Q Science > QR Microbiology > QR180 Immunology Q Science > QR Microbiology > QR355 Virology R Medicine > RB Pathology |
| Divisions: | University Structure - Pre August 2011 > School of Medicine > Infection, Inflammation and Repair |
| Item ID: | 194257 |
| Date Deposited: | 26 Jul 2011 12:23 |
| Last Modified: | 26 Jul 2011 12:23 |
| Contributors: | James, V.L.A. (Author) Lambden, P.R. (Author) Caul, E.O. (Author) Clarke, I.N. (Author) |
| Date: | November 1998 |
| Status: | Published |
| URI: | http://eprints.soton.ac.uk/id/eprint/194257 |
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