Seroepidemiology of human group C rotavirus in the UK
James, V.L.A., Lambden, P.R., Caul, E.O., Cooke, S.J. and Clarke, I.N. (1997) Seroepidemiology of human group C rotavirus in the UK. Journal of Medical Virology, 52, (1), 86-91. (doi:10.1002/(SICI)1096-9071(199705)52:1<86::AID-JMV14>3.0.CO;2-Z). (PMID:9131463).
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Description/Abstract
The gene coding for the major inner capsid protein VP6 of human group C rotavirus was cloned into baculovirus using the pBlueBac2 vector and expressed in insect cells. When cultured in High Five cells, VP6 was expressed at a high level and exported to the cell culture medium. Purified VP6 was used to immunise rabbits. Hyperimmune rabbit serum, which reacted with native human group C rotavirus in infected cells, was used to develop and optimise an EIA for the detection of antibodies to group C rotavirus using the recombinant VP6 as a source of antigen. In a local epidemiological survey of 1000 sera grouped by age, an average of 43% of samples were found to have antibodies to human group C rotavirus with the highest proportion (66%) in the 71-75 year age group. In comparison, 97% of adults and 85% of children had antibodies to recombinant VP6 from the bovine RF strain of group A rotavirus. These results suggest that infection with human group C rotavirus is a common occurrence despite the apparent rarity of reports of human group C rotavirus in clinical samples from patients with gastroenteritis.
| Item Type: | Article |
|---|---|
| ISSNs: | 0146-6615 (print) 1096-9071 (electronic) |
| Keywords: | vp6, baculovirus, elisa, gastroenteritis |
| Subjects: | Q Science > QR Microbiology > QR180 Immunology Q Science > QR Microbiology > QR355 Virology R Medicine > RB Pathology |
| Divisions: | University Structure - Pre August 2011 > School of Medicine > Infection, Inflammation and Repair |
| Item ID: | 194263 |
| Date Deposited: | 26 Jul 2011 12:41 |
| Last Modified: | 26 Jul 2011 12:41 |
| Contributors: | James, V.L.A. (Author) Lambden, P.R. (Author) Caul, E.O. (Author) Cooke, S.J. (Author) Clarke, I.N. (Author) |
| Date: | May 1997 |
| Status: | Published |
| URI: | http://eprints.soton.ac.uk/id/eprint/194263 |
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