Synthesis of a modified thymidine monomer for site-specific incorporation of reporter groups into oligonucleotides
Brown, L. J., May, J. P. and Brown, T. (2001) Synthesis of a modified thymidine monomer for site-specific incorporation of reporter groups into oligonucleotides. Tetrahedron Letters, 42, (13), 2587-2591. (doi:10.1016/S0040-4039(01)00224-6)
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Official URL: http://dx.doi.org/10.1016/S0040-4039(01)00224-6
Description/Abstract
The efficient incorporation of reporter groups into oligonucleotides at specific sites has been facilitated by the synthesis of a novel modified thymidine monomer with an FMOC-protected hydroxyl group on a linker. The primary hydroxyl group can be deprotected during or after solid-phase oligonucleotide synthesis and reacted with any reporter phosphoramidite.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | fluorescence energy-transfer, dye-labeled primers, dna, hybridization, probes |
| Related URLs: | http://dx.doi.org/10.1016/S004...01)00224-6 |
| Subjects: | Q Science Q Science > QD Chemistry |
| Divisions: | University Structure - Pre August 2011 > School of Chemistry |
| ePrint ID: | 19431 |
| URI: | http://eprints.soton.ac.uk/id/eprint/19431 |
| Deposited On: | 14 Feb 2006 |
| Last Modified: | 01 Jun 2011 02:47 |
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