Brown, L.J., May, J.P. and Brown, T.
Synthesis of a modified thymidine monomer for site-specific incorporation of reporter groups into oligonucleotides.
Tetrahedron Letters, 42, (13), . (doi:10.1016/S0040-4039(01)00224-6).
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The efficient incorporation of reporter groups into oligonucleotides at specific sites has been facilitated by the synthesis of a novel modified thymidine monomer with an FMOC-protected hydroxyl group on a linker. The primary hydroxyl group can be deprotected during or after solid-phase oligonucleotide synthesis and reacted with any reporter phosphoramidite.
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