Synthesis of a modified thymidine monomer for site-specific incorporation of reporter groups into oligonucleotides


Brown, L.J., May, J.P. and Brown, T. (2001) Synthesis of a modified thymidine monomer for site-specific incorporation of reporter groups into oligonucleotides. Tetrahedron Letters, 42, (13), 2587-2591. (doi:10.1016/S0040-4039(01)00224-6).

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Description/Abstract

The efficient incorporation of reporter groups into oligonucleotides at specific sites has been facilitated by the synthesis of a novel modified thymidine monomer with an FMOC-protected hydroxyl group on a linker. The primary hydroxyl group can be deprotected during or after solid-phase oligonucleotide synthesis and reacted with any reporter phosphoramidite.

Item Type: Article
ISSNs: 0040-4039 (electronic)
Keywords: fluorescence energy-transfer, dye-labeled primers, dna, hybridization, probes
Subjects: Q Science > QD Chemistry
Divisions: University Structure - Pre August 2011 > School of Chemistry
ePrint ID: 19431
Date Deposited: 14 Feb 2006
Last Modified: 27 Mar 2014 18:09
URI: http://eprints.soton.ac.uk/id/eprint/19431

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