Solution structure of the mEGF/TGF α(44-50) chimeric growth factor
Chamberlin, Stephen G., Brennan, Lorraine, Puddicombe, Sarah M., Davies, Donna E. and Turner, David L. (2001) Solution structure of the mEGF/TGF α(44-50) chimeric growth factor. European Journal of Biochemistry, 268, (23), 6247-6255. (doi:10.1046/j.0014-2956.2001.02581.x)
Download
Full text not available from this repository.
Official URL: http://dx.doi.org/10.1046/j.0014-2956.2001.02581.x
Description/Abstract
The solution structure of the growth factor chimera mEGF/TGF α(44-50) has been determined using an extended version of the DYANA procedure for calculating structures from NMR data. The backbone fold and preferred orientation of the domains of the chimera are similar to those found in previous studies of EGF structures, and several H-bonds used as input constraints in those studies were found independently in the chimera. This shows that the modified activity of the chimera does not result from a major structural change. However, the improved precision of the structure presented here allows the origin of some unusual chemical shifts found in all of these compounds to be explained, as well as the results obtained from some site-specific mutants. Further studies of the properties of this chimeric growth factor should help to elucidate the mechanism(s) of hetero- and homodimerization of the c-erbB receptors.
| Item Type: | Article |
|---|---|
| ISSN: | 0014-2956 (print) |
| Uncontrolled Keywords: | nmr, egf structure, growth factor, indyana, simulated annealing, relaxation matrix approach, factor receptor, factor-α protein structures, tgf-α, biological macromolecules, signal-transduction, chemical-shift, binding |
| Related URLs: | http://www.ncbi.nlm.nih.gov/en...s=11733021 http://dx.doi.org/10.1046/j.00...01.02581.x |
| Subjects: | Q Science > QD Chemistry Q Science > QH Natural history > QH301 Biology |
| Divisions: | University Structure - Pre August 2011 > School of Medicine > Infection, Inflammation and Repair University Structure - Pre August 2011 > School of Chemistry |
| ePrint ID: | 19447 |
| URI: | http://eprints.soton.ac.uk/id/eprint/19447 |
| Deposited On: | 15 Feb 2006 |
| Last Modified: | 02 Mar 2012 12:26 |
Associated Staff Only: edit my ePrint
