On-chip real-time nucleic acid sequence-based amplification for RNA detection and amplification

Tsaloglou, Maria-Nefeli, Bahi, Mahadji M., Waugh, Edward M., Morgan, Hywel and Mowlem, Matthew (2011) On-chip real-time nucleic acid sequence-based amplification for RNA detection and amplification. Analytical Methods, 3 (9), 2127-2133. (doi:10.1039/C1AY05164D).

Abstract

Development of integrated systems for nucleic acid analyses is mainly driven by the requirement for fast and simple clinical and environmental diagnostics. The need for affordable and effective point-of-care diagnosis has inspired an entire field of biotechnology in micro and nano-fluidics. We are developing a microfluidic system that has individual sub-systems for performing cell concentration and lysis, RNA extraction/purification and real-time RNA detection. The system is being developed to analyse the rbcL gene of phytoplankton Karenia brevis, a species responsible for harmful algal blooms. This integrated system will perform sub-cellular analysis of RNA using nucleic acid sequence-based amplification and would be used in large scale biochemical analysis and experimentation. The device could potentially be used for the detection of any species with a known target nucleic acid sequence for in situ environmental monitoring, forensics or clinical diagnostics.

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Contributors

Author: Hywel Morgan

Author: Matthew Mowlem

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