On-chip real-time nucleic acid sequence-based amplification for RNA detection and amplification
Tsaloglou, Maria-Nefeli, Bahi, Mahadji M., Waugh, Edward M., Morgan, Hywel and Mowlem, Matthew (2011) On-chip real-time nucleic acid sequence-based amplification for RNA detection and amplification. Analytical Methods, 3, 2127-2133. (doi:10.1039/C1AY05164D).
Full text not available from this repository.
Development of integrated systems for nucleic acid analyses is mainly driven by the requirement for fast and simple clinical and environmental diagnostics. The need for affordable and effective point-of-care diagnosis has inspired an entire field of biotechnology in micro and nano-fluidics. We are developing a microfluidic system that has individual sub-systems for performing cell concentration and lysis, RNA extraction/purification and real-time RNA detection. The system is being developed to analyse the rbcL gene of phytoplankton Karenia brevis, a species responsible for harmful algal blooms. This integrated system will perform sub-cellular analysis of RNA using nucleic acid sequence-based amplification and would be used in large scale biochemical analysis and experimentation. The device could potentially be used for the detection of any species with a known target nucleic acid sequence for in situ environmental monitoring, forensics or clinical diagnostics.
|Subjects:||G Geography. Anthropology. Recreation > GC Oceanography
G Geography. Anthropology. Recreation > GE Environmental Sciences
Q Science > QA Mathematics > QA75 Electronic computers. Computer science
|Divisions:||Faculty of Physical Sciences and Engineering > Electronics and Computer Science
National Oceanography Centre (NERC) > Ocean Technology and Engineering
|Date Deposited:||18 Aug 2011 08:56|
|Last Modified:||27 Mar 2014 19:45|
|Further Information:||Google Scholar|
|ISI Citation Count:||3|
|RDF:||RDF+N-Triples, RDF+N3, RDF+XML, Browse.|
Actions (login required)