On-chip real-time nucleic acid sequence-based amplification for RNA detection and amplification
Tsaloglou, Maria-Nefeli, Bahi, Mahadji M., Waugh, Edward M., Morgan, Hywel and Mowlem, Matthew (2011) On-chip real-time nucleic acid sequence-based amplification for RNA detection and amplification. Analytical Methods, 3, 2127-2133. (doi:10.1039/C1AY05164D).
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Description/Abstract
Development of integrated systems for nucleic acid analyses is mainly driven by the requirement for fast and simple clinical and environmental diagnostics. The need for affordable and effective point-of-care diagnosis has inspired an entire field of biotechnology in micro and nano-fluidics. We are developing a microfluidic system that has individual sub-systems for performing cell concentration and lysis, RNA extraction/purification and real-time RNA detection. The system is being developed to analyse the rbcL gene of phytoplankton Karenia brevis, a species responsible for harmful algal blooms. This integrated system will perform sub-cellular analysis of RNA using nucleic acid sequence-based amplification and would be used in large scale biochemical analysis and experimentation. The device could potentially be used for the detection of any species with a known target nucleic acid sequence for in situ environmental monitoring, forensics or clinical diagnostics.
| Item Type: | Article |
|---|---|
| ISSNs: | 1759-9660 (print) 1759-9679 (electronic) |
| Subjects: | G Geography. Anthropology. Recreation > GC Oceanography G Geography. Anthropology. Recreation > GE Environmental Sciences Q Science > QA Mathematics > QA75 Electronic computers. Computer science |
| Divisions: | Faculty of Physical Sciences and Engineering > Electronics and Computer Science National Oceanography Centre (NERC) > Ocean Technology and Engineering |
| Item ID: | 195275 |
| Date Deposited: | 18 Aug 2011 08:56 |
| Last Modified: | 22 May 2013 01:01 |
| Contributors: | Tsaloglou, Maria-Nefeli (Author) Bahi, Mahadji M. (Author) Waugh, Edward M. (Author) Morgan, Hywel (Author) Mowlem, Matthew (Author) |
| Date: | 17 August 2011 |
| Status: | Published |
| Further Information: | Google Scholar |
| ISI Citation Count: | 3 |
| URI: | http://eprints.soton.ac.uk/id/eprint/195275 |
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