Analysis of photosynthetic picoeukaryote community structure along an extended Ellett Line transect in the northern North Atlantic reveals a dominance of novel prymnesiophyte and prasinophyte phylotypes


Kirkham, Amy R., Jardillier, Ludwig E., Holland, Ross, Zubkov, Mikhail V. and Scanlan, Dave J. (2011) Analysis of photosynthetic picoeukaryote community structure along an extended Ellett Line transect in the northern North Atlantic reveals a dominance of novel prymnesiophyte and prasinophyte phylotypes. Deep Sea Research Part I Oceanographic Research Papers, 58, (7), 733-744. (doi:10.1016/j.dsr.2011.05.004 ).

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Description/Abstract

Photosynthetic picoeukaryotes (PPEs) of a size <3 μm can contribute significantly to primary production. Here, PPE community structure was analysed along an extended Ellett Line transect, an area in the North Atlantic well studied by physical oceanographers but largely neglected in the field of microalgal ecology. Distribution patterns of specific PPE classes were determined using dot-blot hybridization analysis, while the taxonomic composition of specific PPE classes was revealed by phylogenetic analysis of plastid 16S rRNA gene sequences. In addition, we performed fluorescent in situ hybridization (FISH) analysis of seawater samples collected along the transect to provide a PCR-independent survey of class level PPE distribution patterns. We found the PPE community was dominated by members of the Prymnesiophyceae, Prasinophyceae and Mamiellophyceae. Interestingly, phylogenetic analysis revealed several novel Prymnesiophyceae and Prasinophyceae phylotypes (with only 85–96% identity to neighbouring sequences) within lineages for which cultured counterparts are unknown.

Item Type: Article
ISSNs: 0967-0637 (print)
Keywords: Photosynthetic picoeukaryotes; Community structure; Prymnesiophytes; Prasinophytes
Subjects: G Geography. Anthropology. Recreation > GC Oceanography
Q Science > QH Natural history > QH301 Biology
Divisions: National Oceanography Centre (NERC) > Ocean Biogeochemistry and Ecosystems
ePrint ID: 195759
Date Deposited: 26 Aug 2011 10:47
Last Modified: 27 Mar 2014 19:45
URI: http://eprints.soton.ac.uk/id/eprint/195759

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